Amphotericin b solution

Minimum Essential Medium (MEM) was purchased from Capricorn Scientific GmbH (Ebsdorfergrund, Germany), while DMEM/F12 medium, penicillin-streptomycin solution, amphotericin B solution, L-glutamine and trypsin were purchased from Biowest (Nuaillé, France). Fetal bovine serum (FBS), non-essential amino acids, DMSO, Thiazolyl blue tetrazolium bromide (MTT) and JC-1 were obtained from Sigma-Aldrich Chemie GmbH. Dihydroethidium (DHE), dihydrorhodamine 123 (DHR), fluorescein-di-β-D-galacto-pyranoside (FDG) and hoechst 33342 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Annexin-V-FITC (AV) and propidium iodide (PI) Apoptosis Detection Kit was purchased from Abcam (Cambridge, UK). Bovine serum albumin (BSA) was from Serva (Heidelberg, Germany) and Triton™ X-100 was from Merck KGaA (Darmstadt, Germany). Anti-phospho-histone H2A.X (Ser¹³⁹) rabbit primary antibody was from Cell Signaling Technology^® (Danvers, MA, USA) and Alexa Fluor^® 488 goat anti-rabbit IgG (H + L) secondary antibody was purchased from Thermo Fisher Scientific.

The Amphotericin B solution (product code L0009) was purchased from Biowest (VWR International GmbH, Darmstadt, Germany). Filipin III (from Streptomyces filipinensis, product code F4767), natamycin (product code 32417), and sodium deoxycholate (product code D6750) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Nystatin (product code 15340029) was purchased from Gibco (Thermo Fisher Scientific, Waltham, Massachusetts, United States). Ethanol (product code 9065.4) and mEthanol (CP43.3) were purchased from Carl Roth (GmbH + Co., KG, Karslruhe, Germany).