Tcf lef h2b gfp transgenic mice

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TCF/Lef:H2B-GFP transgenic mice express green fluorescent protein (GFP) under the control of the TCF/Lef transcriptional response element. This model can be used to visualize Wnt/β-catenin signaling activity in live cells and tissues.

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Lab products found in correlation

C3h mice, by Charles River Laboratories (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Lyz2 cre, by Jackson ImmunoResearch (1 mentions) Ctnnb1fl fl, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

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TCF/Lef:H2B/GFP mice (6 citations) TCF/Lef:H2B-GFP (6 citations) H2B-GFP mice (3 citations)

3 protocols using tcf lef h2b gfp transgenic mice

Investigating Wnt Signaling in Mice

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All animal procedures for this study were approved by the Institutional Animal Care and Use Committee (IACUC) at Duke University. Experiments were carried out with mice that were between 8 to 12 weeks old and contemporaneous vehicle controls were used. C57Bl/6 mice were purchased from Jackson Laboratories (stock number 000664) and C3H mice were purchased from Charles River (catalog number 25). The TCF/Lef:H2B-GFP transgenic mice were purchased from Jackson laboratories (stock number 013752) and bred in-house. SB216763 was administered in 100% DMSO at the indicated dose by subcutaneous injection.

Daniel A.R., Lee C.L., Oh P., Luo L., Ma Y, & Kirsch D.G. (2020). Inhibiting glycogen synthase kinase-3 mitigates the hematopoietic acute radiation syndrome in a sex and strain dependent manner in mice. Health physics, 119(3), 315-321.

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Macrophage Depletion and Wnt Modulation in Mice

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Experiments were performed with 6- to 12-week old Crl:CD1 (ICR) and FVB/N female mice. Mice were sacrificed at specific postnatal days (P), and their dorsal skins were dissected and processed for analyses. To reduce the number of skin-resident macrophages, 1 mg of clodronate-encapsulated liposomes were administered to mice via daily subcutaneous injections during two alternated days (Encapsula Nanosciences). CL-lipo are the one of the most effective, specific, and extensively used agents to deplete phagocytic monocytes and macrophages via apoptosis [49] (link),[53] (link). The specific Wnt inhibitor IWP-2 (Roche Diagnostics) was encapsulated in liposomes (Encapsula Nanosciences) and 50 µg were injected subcutaneously [75] (link),[78] (link). The K5 tTA(TetOff)-histone H2B-GFP mice [17] (link), the K15-GFP mice (Jackson Lab) [103] (link), the Katushka reporter mice [108] (link), and the TCF/Lef:H2B-GFP transgenic mice (Jackson Lab) [67] (link) have been previously described. Doxycycline treatments were initiated in 28 d postnatal mice [17] (link), and maintained until the collection of samples after the performance of subcutaneous injections of CL-lipo and Lipo at specified times.

Castellana D., Paus R, & Perez-Moreno M. (2014). Macrophages Contribute to the Cyclic Activation of Adult Hair Follicle Stem Cells. PLoS Biology, 12(12), e1002002.

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Genetically Engineered Mouse Models for Research

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C57BL/6J, Lyz2-cre⁺ (Jax#019096), Rspo3^fl/fl (Jax#027313), Ctnnb1^fl/fl (022775), Tet2^fl/fl (017573), TCF/Lef:H2B-GFP transgenic mice (Jax#013752), and Rosa26-floxed STOP-Cas9 mice (Jax#024857) were originally purchased from Jackson Laboratory. VE-cadherin-CreERT2 mice (MGI#3848982) were kindly provided by Dr. Ralf Adams. Rspo3^fl/fl mice were crossed with VE-cadherin-CreERT2 mice to generate VE-cadherin-CreERT2⁺;Rspo3^fl/fl mice (Rspo3^EC−/−), Ctnnb1^fl/fl and Tet2^fl/fl mice were crossed with Lyz2-cre⁺ mice to generate Lyz2-cre⁺;Ctnnb1^fl/fl mice (Ctnnb1^Mφ−/−) and Lyz2-cre⁺;Tet2^fl/fl mice (Tet2^Mφ−/− ), respectively. Genotyping of these mice strains were either done by regular PCR using the recommended primers in Jackson Laboratory website (www.jax.org) followed by DNA gel imaging or by Transnetyx Inc (Cordova, TN, USA) using the TaqMan probe-based qPCR. All mice were housed in a temperature-controlled specific pathogen-free facility under 12-h light/dark cycles in the University of Illinois at Chicago Animal Care Facility. Veterinary care and animal experimental procedures were approved by the University of Illinois Animal Care & Use Committee in accordance with the guidelines of the National Institutes of Health.

Zhou B., Magana L., Hong Z., Huang L.S., Chakraborty S., Tsukasaki Y., Huang C., Wang L., Di A., Ganesh B., Gao X., Rehman J, & Malik A.B. (2020). The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injury. Nature immunology, 21(11), 1430-1443.

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