Ab108842

Manufactured by Abcam

Sourced in United Kingdom, Japan

Ab108842 is a rabbit monoclonal antibody that recognizes human Vimentin. It is suitable for use in Western Blotting, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), and Immunocytochemistry/Immunofluorescence applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab277386, by Abcam (1 mentions) Human tnf α quantikine elisa, by R&D Systems (1 mentions) Human fibrinogen elisa kit, by Abcam (1 mentions) Vacuette, by Greiner (1 mentions) Serum triglyceride determination kit, by Merck Group (1 mentions) Ranbut, by Randox (1 mentions)

2 protocols using ab108842

Biochemical Profiling of Bovine Serum Markers

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The serum activity of aspartate aminotransferase (AST, catalog No.; AS101) was estimated using kits obtained from the Randox company (Kearneysville, VA, USA). Lactate dehydrogenase (LDH, catalog No.; TK41214), total protein (Catalog No.; MD1001291), and albumin (Catalog No.; MX1001020) were assessed using kits obtained from the Spinreact company (Santa Coloma, Spain) according to the described methods of its manufacturer’s instructions.
Serum levels of acute-phase protein (APP) including haptoglobin (Catalog No.; ab137977), amyloid A (Catalog No.; ab274407), fibrinogen (Catalog No.; ab108842), ceruloplasmin (Catalog No.; ab108818), and ferritin (Catalog No.; ab108698) were estimated using kits obtained from the Abcam company (Cambridge, UK) according to the standard protocol of their specific pamphlets.
Serum tumor necrosis factor-alpha (TNF-α, Catalog No.; MBS2609886), interleukins, IL-1β, IL-6, (Catalog No.; # ESS0027, and # RBOIL6I) (Invitrogen-Thermo Fisher Co., Waltham, MA, USA) and IL-10, (Catalog No.; ab277386) (Abcam Co., Cambridge, UK) levels were estimated using specific bovine ELISA commercial kits according to the methodology of each enclosed pamphlet. All parameters were measured spectrophotometrically using a 5010 Photometer (ROBERT RIELE GmbH & Co KG, Berlin, Germany).

Sadat A., Farag A.M., Elhanafi D., Awad A., Elmahallawy E.K., Alsowayeh N., El-khadragy M.F, & Elshopakey G.E. (2023). Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by Escherichia coli and Staphylococcus aureus Infection. Animals : an Open Access Journal from MDPI, 13(5), 892.

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Postprandial Metabolic and Inflammatory Markers

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At each time point, 10 mL of venous whole blood was taken and dispensed into serum separation and lithiumheparin (Vacuette; Greiner Bio-One GmbH, Kremsmünster, Austria) tubes before being centrifuged for 15 min at 2000g at 4°C and stored at -80°C for retrospective analysis of triglycerides (TGs) (Serum Triglyceride Determination Kit; Sigma-Aldrich, St. Louis, MO, USA). Apolipoprotein B48 (APO B48 ; Antibodies-online, USA), non-esterified-fatty acids (NEFA) (RANBUT; Randox Laboratories, London, UK), plasma glucagon (Glucagon EIA; Sigma-Aldrich) and tumour necrosis factor alpha (TNF-α) (Human TNF-α Quantikine ELISA; R&D Systems, Roche Diagnostics, West Sussex, UK) were measured hourly. Plasma fibrinogen (ab108842, Fibrinogen Human ELISA Kit; Abcam, Japan), HTF (Human Tissue Factor activity ab108906; Abcam, UK) and PAI-1 (Human PAI-1/serpin ELISA Kit DSE100; R&D systems, UK) were measured at rest, 3 h and 6 h postmeal. The intra-assay coefficient of variation was <10% for all assays. Due to increased assay cross-reactivity with insulin detemir, only participants treated with insulin glargine were included in serum insulin analysis (n = 8).

Campbell M.D., Walker M., Ajjan R.A., Birch K.M., Gonzalez J.T, & West D.J. (2017). An additional bolus of rapid-acting insulin to normalise postprandial cardiovascular risk factors following a high-carbohydrate high-fat meal in patients with type 1 diabetes: A randomised controlled trial. Diabetes & vascular disease research, 14(4).

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