Vanillin was purchased Shanghai Sangon Biological Engineering Technology (Shanghai, China). o-Toluidine and N, N-dimethylformamide were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China). Sodium citrate tribasic dihydrate and Tris were purchased from Sigma-Aldrich (Steinheim, Germany). Glycine was purchased from GenStar Biosolutions Co., Ltd. (Beijing, China). Citric acid, disodium hydrogen, HCl, NaOH, Na2HPO4 and acetic acid were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). Acetonitrile was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). n-Hexane was purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). Other chemicals were purchased from standard commercial sources and were of analytical grade purity.
Citric acid
Manufactured by Guangzhou Chemical
Sourced in China
Citric acid is a white crystalline solid with a sharp, acidic taste. It is a naturally occurring organic acid found in citrus fruits. Citric acid is a weak acid that is commonly used in food, beverage, and pharmaceutical industries as a flavoring agent, preservative, and pH regulator.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Guangzhou Chemical (2 mentions)
Sodium hydroxide (naoh), by Guangzhou Chemical (2 mentions)
Acetic acid, by Guangzhou Chemical (1 mentions)
Sodium citrate tribasic dihydrate, by Merck Group (1 mentions)
Acetonitrile, by Tianjin Damao (1 mentions)
Na2hpo4, by Guangzhou Chemical (1 mentions)
N hexane, by Chron Chemicals (1 mentions)
Vanillin, by Sangon (1 mentions)
Ethyl acetate, by Guangzhou Chemical (1 mentions)
Cacl2, by Guangzhou Chemical (1 mentions)
Trypsin, by Solarbio (1 mentions)
K2s2o8, by Guangzhou Chemical (1 mentions)
Pepsin, by Solarbio (1 mentions)
Hexane, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Phospholipid standards, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Sangon (1 mentions)
Pentobarbital, by Merck Group (1 mentions)
4 protocols using citric acid
1
Detailed Reagent Procurement Protocol
2
Curcumin and Bile Powder Extraction Protocol
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Curcumin (purity ＞95%) was purchased from Kermel Chemical Reagent Co., Ltd (Tianjin, China), and bile powder (from pig) was purchased from Sigma Chemical Co., Ltd (St. Louis, MO, USA). GA was purchased from Dingli Gum Industry Co., Ltd (Tai’an, China). Pepsin (1:10000) and trypsin (1:250) were obtained from Solar Bio Science & Technology Co., Ltd. (Beijing, China). Other reagents (NaOH, HCl, CaCl2, K2S2O8, absolute alcohol, ethyl acetate, and citric acid) of analytical grade were purchased from Guangzhou chemical reagent factory (Guangzhou, China).
3
Fatty Acid Profiling of Chilled Mackerel
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Chilled mackerel was purchased from Hailan Kitchen Co., Ltd. (Haikou, China). Methanol (chromatographically pure), chloroform (analytically pure), isopropanol (chromatographically pure), hexane (chromatographically pure), 4-methyl umbel ketone (>99%), phospholipid standards, linoleic acid standard (>99%), and 37 types of fatty acid methyl ester mixed standards were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Phosphoric acid buffer (pH = 7.0) and citric acid (analytically pure) were bought from Guangzhou Chemical Reagent Factory (Guangzhou, China). N-alkanes (C6-C40) were purchased from o2si Biotechnology Co., Ltd. (Beijing, China). Acetic acid, ethyl ether, n-heptane, 2,4,6-trimethylpyridine (TMP), potassium hydroxide, Ethylene Glycol Tetra-acetic Acid (EGTA), dithiothreitol (DTT), ethylenediamine tetra-acetic acid (EDTA), and disodium hydrogen phosphate (Na2HPO4) were purchased from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China).
4
SCID Mouse Model for Angiogenesis Study
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A total of 30 female specific pathogen free (SPF) CB17 severe combined immunodeficiency (SCID) mice (weight, 17.02±0.75 g; age, 6–7 weeks) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). RAPA was purchased from LC Laboratories (Woburn, MA, USA). Mouse HIF-1α and VEGF ELISA kits (cat. nos. CSB-E08541m and CSB-E04756m, respectively) were obtained from Sino-American Biotechnology Co., Ltd. (Wuhan, China). Mouse anti-human HIF-1α polyclonal antibody (1:100; cat. no. BM0912) was purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). Mouse anti-human VEGF (1:200; cat. no. RB-9031) and CD34 (1:100; cat. no. MS-363) monoclonal antibodies, streptomycin avidin-peroxidase (SP) immunochemistry (cat. no. Kit-9701) and DAB (cat. no. Kit-0017) kits were from Maxim Biotech, Inc. (Fuzhou, China). Lidocaine and pentobarbital were obtained from Sigma-Aldrich (St. Louis, MO, USA). Formalin, paraffin and bovine serum albumin (BSA) Sangon Biotech, Co., Ltd. (Shanghai, China). Citric acid and sodium citrate were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China).
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