The MTT Cell Proliferation Kit was purchased from the Beyotime Institute of Biotechnology. Experiments were carried out according to the manufacture’s recommendation. The absorbance at 540 nm was measured using a microplate reader (Thermo Fisher Scientific).
Mtt cell proliferation kit
Manufactured by Beyotime
Sourced in China
The MTT cell proliferation kit is a laboratory tool used to measure the viability and proliferation of cells in culture. It is based on the reduction of the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by metabolically active cells, resulting in the formation of purple formazan crystals that can be quantified spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Hydrogen peroxide h2o2, by Sinopharm (1 mentions)
Microplate reader, by Tecan (1 mentions)
Ldh cytotoxicity assay kit, by Beyotime (1 mentions)
Elx800, by Agilent Technologies (1 mentions)
Easypure mirna kit, by Transgene (1 mentions)
Facscanto flow cytometer, by BD (1 mentions)
Transscript green mirna two step qrt pcr supermix, by Transgene (1 mentions)
Mitomycin c, by Merck Group (1 mentions)
T cell enrichment column, by R&D Systems (1 mentions)
7 protocols using mtt cell proliferation kit
1
MTT Cell Proliferation Assay
2
MTT Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation or viability was measured using an MTT cell proliferation kit (Beyotime Biotechnology, Shanghai, China) following the manufacturer’s instructions. Briefly, cells were seeded into 96-well plates at a density of 5 × 104 cells per well and were cultured for 12 h. Then, cells were infected with live/heat-killed bacteria or treated with CM for the indicated times. The absorbance was measured at 570 nm by an iMark Microplate Absorbance Reader.
3
Milk Powder and Taibi Flour Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The whole milk powder was purchased from Yili Industrial Group Co., Ltd. (Hohhot, China). TB was purchased from Chengdu Jintiankang Food Factory (Chengdu, China) and ground into flour (200 mesh) by a mill (Bear Electric Appliance Co., Ltd., Foshan, China). Sodium hydroxide (analytical reagent, AR), sodium chloride (AR), phenolphthalein (AR), and hydrogen peroxide (H2O2) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). De Man-Rogosa-Sharpe (MRS) and modified Chalmers (MC) medium were purchased from Qingdao Rishui Bio-technologies Co., Ltd. (Qingdao, China). IPEC-J2 cells were donated from the laboratory of food science and engineering in Wuhan Polytechnic University. Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were bought from Gibco Company (Grand Island, NY, USA). Beyotime Company (Haimen, China) provided the 3-(4,5-dimehthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation kit and cytotoxicity assay kit.
4
Evaluating Homocysteine-Induced Cytotoxicity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was assessed by using the MTT Cell Proliferation kit (Beyotime). Briefly, H9c2 cardiac cells were passaged in 96-well plates and treated with 100 μM homocysteine for 72 h. Then the cells were cultured in fresh culture medium with 0.5 mg/mL of MTT solution for 1 h at 37°C. Subsequently the amount of formazan was dissolved in DMSO and measured with a microplate reader (Tecan, Mannedorf, Switzerland) at 570 nm as an index of cell viability.
Cell cytotoxicity was evaluated by using the LDH Cytotoxicity Assay kit (Beyotime) according to the manufacturer’s instructions. Briefly, cells were passaged in 96-well plates and treated with 100 μM homocysteine for 72 h. The release of LDH in the medium (LDHm) and in the cell extract (LDHc) of each well was measured with a microplate reader (Tecan) at 490/620 nm. Cytotoxicity was defined as the ratio of LDHm/(LDHm+LDHc)×100%.
Cell cytotoxicity was evaluated by using the LDH Cytotoxicity Assay kit (Beyotime) according to the manufacturer’s instructions. Briefly, cells were passaged in 96-well plates and treated with 100 μM homocysteine for 72 h. The release of LDH in the medium (LDHm) and in the cell extract (LDHc) of each well was measured with a microplate reader (Tecan) at 490/620 nm. Cytotoxicity was defined as the ratio of LDHm/(LDHm+LDHc)×100%.
5
Quantitative miRNA Profiling in Cell Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Main instruments and reagents were as follows: quantitative fluorescence polymerase chain reaction (PCR) (7500; Applied Biosystems, Foster City, USA), total RNA extraction kit (EasyPure miRNA Kit; Transgen, Beijing, China). PCR-reverse transcription kit (TransScript ® Green miRNA Two-Step qRT-PCR SuperMix; TransGen Biotech, Beijing, China), Lipofectamine 2000 and Annexin V-FITC cell apoptosis kit (Invitrogen, Carlsbad, USA), incubator (RPMI-1640; Hyclone, Logan, USA), rabbit antihuman TBX1 primary antibody (Abcam, Cambridge, UK), rabbit anti-human occludin polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, USA), mouse antihuman β-catenin primary antibody (Boster Biological Technology, Wuhan, China), miR-122a mimics, miR-122a NG, miR-3195 mimics, and miR-3195 NG (Sangon Biotech, Shanghai, China), horseradish peroxidase (HRP)conjugated goat anti-mouse secondary antibody, MTT cell proliferation kit (Beyotime Biotechnology, Shanghai, China), FACSCanto flow cytometer (Becton Dickinson, Franklin Lakes, USA), and a microplate reader (Elx-800; BioTek, Winooski, USA). Primers of miR-122a, miR-3195 and U6 were designed and synthesized by Shanghai Ge-nePharma Co., Ltd (Shanghai, China) (Table 1).
6
Bacterial-Induced Cell Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTT cell proliferation kit (Beyotime Biotechnology, China) was used to measure cellular viability and proliferation according to the instructions of the manufacturer. Briefly, cells at the density of 1 × 105 each well were seeded in 96-well plates. It was followed by infection of the cells with bacteria for indicated durations of time (24 h and 48 h). A microplate reader (Thermo Scientific, USA) was used to measure the absorbance at 570 nm.
7
Immethridine-treated DC-mediated Allogeneic T Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T cells were purified from splenocytes of BALB/c mouse using T cell Enrichment Columns (R&D Systems, Minneapolis, MN) and were used as responders (1×105/well). The bone marrow-derived DCs from C57BL/6 mice were prepared as before. The harvested DCs were treated with immethridine (1 μM) or PBS for 4 h in a humidified atmosphere with 5% CO2 at 37 °C. Then DCs were treated with 30 μg/ml Mitomycin C (Sigma, USA) for 45 min. After being washed 3 times by RPMI 1640, they were used as stimulators. For the induction of allogeneic MLR, purified T cells were co-cultured with immethridine-treated or untreated allogeneic DCs at the DC/T cell ratio of 1:5, 1:10 and 1:20 in triplicate in flat-bottom 96-well microplates. Negative control received only T cells and media. The plates were incubated at 37 °C in a humid atmosphere with 5% CO2 for 5 days and the result was measured by MTT Cell Proliferation Kit (Beyotime, China) according to the manufacturer’s instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!