Mouse experiments were conducted in accordance with Institutional Animal Care and Committee (IACUC) protocols and Animal Resource Center (ARC) at Case Western Reserve University. Cre-ERT2 and Pten knockout mice were obtained from Jackson Laboratory. Sprr2f-Cre mice were provided by Dr. Leone. Cyclin D1Flox/WT mice were generated in Cyagen Biosciences Inc. Tamoxifen (Sigma) was administered orally to Cyclin D1Flox/WT Cre-ERT2 once a day for 5 days at a dose of 200mg/kg of body weight to generate Cyclin D1TA/WT mice. 1–2 mg of BrdU was administered by intraperitoneal injection two hours prior to analysis. The genotypes and DNA recombination were verified by genomic PCR. PCR primers are as follows: cyclin D1Flox/WT F1: 5’-GTGGAAGGCTTCCAGGTGTGAC-3’, R1: 5’-CCTAGTATGGACCCACAGACACCC-3’, F3: 5’-GCCCCATTCCTACATATCTCA-3’ and R3: 5’-TGGATCCACCTAATAACTTCGTA-3’.
Pten knockout mice
Manufactured by Jackson ImmunoResearch
Pten knockout mice are genetically modified mice that lack the Pten gene, which is a tumor suppressor gene. These mice are used in research to study the role of Pten in various biological processes and disease models.
Automatically generated - may contain errors
2 protocols using pten knockout mice
1
Conditional Cyclin D1 Deletion Mice
2
Conditional Cyclin D1 Deletion Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse experiments were conducted in accordance with Institutional Animal Care and Committee (IACUC) protocols and Animal Resource Center (ARC) at Case Western Reserve University. Cre-ERT2 and Pten knockout mice were obtained from Jackson Laboratory. Sprr2f-Cre mice were provided by Dr. Leone. Cyclin D1Flox/WT mice were generated in Cyagen Biosciences Inc. Tamoxifen (Sigma) was administered orally to Cyclin D1Flox/WT Cre-ERT2 once a day for 5 days at a dose of 200mg/kg of body weight to generate Cyclin D1TA/WT mice. 1–2 mg of BrdU was administered by intraperitoneal injection two hours prior to analysis. The genotypes and DNA recombination were verified by genomic PCR. PCR primers are as follows: cyclin D1Flox/WT F1: 5’-GTGGAAGGCTTCCAGGTGTGAC-3’, R1: 5’-CCTAGTATGGACCCACAGACACCC-3’, F3: 5’-GCCCCATTCCTACATATCTCA-3’ and R3: 5’-TGGATCCACCTAATAACTTCGTA-3’.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!