1 2 ethanediol

Manufactured by Merck Group

Sourced in United States

1,2-ethanediol is a clear, colorless, viscous liquid chemical compound. It is commonly used as a raw material in the production of various chemical products and as a component in antifreeze solutions.

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Ethanediol (2 citations)

6 protocols using 1 2 ethanediol

Synthesis of Pure LaFeO3 by Calcination

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Pure LaFeO₃ was obtained through the calcination in the temperature range of 550 to 800 °C of the LaFe(C₂O₄)₃·3H₂O precursor, which was obtained previously. It was used as reagents: La(NO₃)₃·6H₂O, (Fe(NO₃)₃·9H₂O, 1,2-ethanediol, 2 M HNO₃ solution from Merck (Darmstadt, Germany). NaOH and capecitabine (CCB) used was an analytical-grade reagent from Merck. The doubly distilled and deionised water were used for preparing all solutions.

Dumitru R., Negrea S., Ianculescu A., Păcurariu C., Vasile B., Surdu A, & Manea F. (2020). Lanthanum Ferrite Ceramic Powders: Synthesis, Characterization and Electrochemical Detection Application. Materials, 13(9), 2061.

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Osteoprotective Effects of Methylprednisolone

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Methylprednisolone (MP), calcein, hematoxylin, eosin, oleic acid, tocopherol succinate, ethylene glycol 1000 were purchased from Sigma-Aldrich (USA). 1,2 ethanediol was purchased from Merck Millipore (USA) and Tween80 was purchased from Himedia (India). PINP and CTX-1 ELISA kits were purchased from MyBioSource (USA). All primers were purchased from IDT (USA). MicroRNA assay system was purchased from Invitrogen (USA). Atrogin 1 antibody (ab168372) was purchased from Abcam (UK), MuRF 1 antibody (sc-32920) was purchased from Santa Cruz (USA) and GAPDH antibody (MAB374) was purchased from Merck Millipore (USA)^{14 (link)}.

Pal S., Mittapelly N., Husain A., Kushwaha S., Chattopadhyay S., Kumar P., Ramakrishna E., Kumar S., Maurya R., Sanyal S., Gayen J.R., Mishra P.R, & Chattopadhyay N. (2020). A butanolic fraction from the standardized stem extract of Cassia occidentalis L delivered by a self-emulsifying drug delivery system protects rats from glucocorticoid-induced osteopenia and muscle atrophy. Scientific Reports, 10, 195.

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Liposome Preparation and Characterization

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DOPC was obtained from Avanti Polar Lipids. MDM with block molecular weights of 900-4800-900 Da and M_w/M_n of 1.23, and PB-PEO with block molecular weights of 2500-900 Da and M_w/M_n of 1.09 were obtained from Polymer Source Inc. 5(6)-CF, propionic acid, and butyric acid were obtained from Acros. M Ethanol, 1-propanol, boric acid, magnesium sulfate heptahydrate, magnesium chloride hexahydrate, and hydrochloric acid were obtained from J.T. Baker. Ethanol, 1-butanol, 1,2-ethanediol, 1,3-propanediol, 1,4-butanediol, 1,5-pentanediol, erythritol, xylitol, mannitol, urea, sodium chloride, Hepes, and acetic acid were obtained from Sigma-Aldrich. Glycerol and tris were obtained from American Bioanalytical. Triton X-100 was obtained from Thermo Fisher Scientific. Sodium hydroxide was obtained from Macron.

Werber J.R, & Elimelech M. (2018). Permselectivity limits of biomimetic desalination membranes. Science Advances, 4(6), eaar8266.

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Synthesis of α-Conotoxin Analogs

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The linear RgIA (GCCSDPRCRYRCR) and its analogs were folded in two steps to facilitate the formation of intramolecular disulfide bonds between Cys2 and Cys8 and between Cys3 and Cys12, forming the 1–3, 2–4 disulfide arrangement that is characteristic of α-conotoxins. The linear peptide was cleaved from the Wang resin using reagent K (trifluoroacetic acid (TFA) (Fisher Bioreagents), phenol (Arcos Organics), 1,2-ethanediol (Sigma Aldrich), thioanisole (Sigma Aldrich), H₂O / 9.0:0.75:0.25:0.5:0.5 ratio by volume). The first oxidation step to close Cys2-Cys8 bridge was performed under potassium ferricyanide (4.0 mM) (Arcos Organics) and tris HCl (20 mM) (Mallinckrodt Pharmaceuticals, Surrey, United Kingdom), pH 7.5 for 45 minutes and purified via reversed-phase high-performance liquid chromatography (RP-HPLC) using a C18 column. The second disulfide bridge between Cys3-Cys12 was formed in the solution consisting of iodine (5.0 mM) in H₂O, acetonitrile (ACN) (Fisher Chemical), and TFA (72.0:25.0:3.0 ratio by volume) and again, purified through RP-HPLC.

Huynh P.N., Harvey P.J., Gajewiak J., Craik D.J, & Michael McIntosh J. (2020). Critical Residue Properties for Potency and Selectivity of α-Conotoxin RgIA Towards α9α10 Nicotinic Acetylcholine Receptors. Biochemical pharmacology, 181, 114124.

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Solid-Phase Peptide Synthesis of Customized Proteins

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Trifluoroacetic acid (TFA), piperidine, thioanisole, 1,2- Ethanediol, diethylether, N,N′-dimethylformamide (DMF), Diisopropylethyl amine (DIPEA), rink amide resins, 1-Hydroxybenzotriazole (HOBt), and O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU) were obtained from Sigma Aldrich, N-α-Fmoc protected amino acids were obtained from Novabiochem (Merck, United States) and bacterial media was obtained from Himedia^TM (India). 6X-Histidine and FLAG-LPETG peptide were purchased from Genscript Biotech (United States). All antibodies for ELISA and Confocal microscopy were purchased from Invitrogen. ProLong Gold antifade was purchased from Thermo Fisher Scientific. The experimental bacterial strains, Staphylococcus aureus (MTCC 3160), Enterococcus faecalis (MTCC 3159) and Escherichia coli (MTCC 42) were procured from Microbial Type Culture Collection (IMTECH, Chandigarh, India).

Kumari P., Nath Y., Murty U.S., Ravichandiran V, & Mohan U. (2020). Sortase A Mediated Bioconjugation of Common Epitopes Decreases Biofilm Formation in Staphylococcus aureus. Frontiers in Microbiology, 11, 1702.

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Synthesis of Zinc Acetate Thin Films

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Zinc acetate dihydrate [Zn(CH 3 COO) 2 Á2H 2 O, Aldrich >99.5%], ethyl acetoacetate (CH 3 COCH 2 COOC 2 H 5 , Aldrich >99%), Pluronic F127 (PEO 106 -PPO 70 -PEO 106 , Aldrich), 2propanol (Carlo Erba 99.7%), ethanol (Fluka >99.8% EtOH) and 1,2-ethanediol (ethylene glycol; Sigma-Aldrich 99.8%) were used as received without further purification; bidistilled water (H 2 O) was employed in the synthesis. p-Type borondoped silicon wafers were employed as the substrates for film deposition.

Malfatti L., Pinna A., Enzo S., Falcaro P., Marmiroli B, & Innocenzi P. (2015). Tuning the phase transition of ZnO thin films through lithography: an integrated bottom-up and top-down processing. Journal of synchrotron radiation, 22(1).

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