Nutrient supplemented plates for paralysis assays contained 10mM D(+)-glucose (Fisher Scientific), 0.3mM sodium homogamma linolenate (Nu-Chek Prep), 0.1mM or 0.3mM oleic acid (ThermoFisher Scientific),148nM or 740 nM methylcobalamin (Millipore Sigma), 1.34 mM, 6.7 mM, 13.4mM, or 67 mM L-methionine (Fisher Scientific), 1 mM, 10 mM, or 20 mM choline chloride (Millipore Sigma), and 15mM or 30 mM DL-Homocysteine (Millipore Sigma). Supplements were added to autoclaved NGM media at 55°C. Specific concentrations used for each supplementation experiment are indicated in the figures and legends. Some of these concentrations for glucose (Alcántar-Fernández et al., 2018 (link)), fatty acids (Deline et al., 2013 ), methylcobalamin (Revtovich et al., 2019 (link)), L-methionine (Wei and Ruvkun, 2020 (link)), homocysteine (Wei and Ruvkun, 2020 (link)), and choline (Walker et al., 2011 (link)) were used in prior C. elegans work; additional concentrations were used to determine dose-dependent effects for certain treatments. Plates were stored at 4°C. Fatty acid supplemented plates were covered with foil to prevent light oxidation. Plates were seeded with the different E. coli cultures (OD600 = 1.0) and dried for three days before use.
Sodium homogamma linolenate
Manufactured by Nu-Chek Prep
Sodium homogamma linolenate is a laboratory chemical compound. It is a salt of the omega-6 fatty acid gamma-linolenic acid.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sodium homogamma linolenate
1
Nutrient Supplementation for Paralysis Assays
2
Nutrient Supplementation for Paralysis Assays
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Nutrient supplemented plates for paralysis assays contained 10mM D(+)-glucose (Fisher Scientific), 0.3mM sodium homogamma linolenate (Nu-Chek Prep), 0.1mM or 0.3mM oleic acid (ThermoFisher Scientific),148nM or 740 nM methylcobalamin (Millipore Sigma), 1.34 mM, 6.7 mM, 13.4mM, or 67 mM L-methionine (Fisher Scientific), 1 mM, 10 mM, or 20 mM choline chloride (Millipore Sigma), and 15mM or 30 mM DL-Homocysteine (Millipore Sigma). Supplements were added to autoclaved NGM media at 55°C. Specific concentrations used for each supplementation experiment are indicated in the figures and legends. Some of these concentrations for glucose (Alcántar-Fernández et al., 2018 (link)), fatty acids (Deline et al., 2013 ), methylcobalamin (Revtovich et al., 2019 (link)), L-methionine (Wei and Ruvkun, 2020 (link)), homocysteine (Wei and Ruvkun, 2020 (link)), and choline (Walker et al., 2011 (link)) were used in prior C. elegans work; additional concentrations were used to determine dose-dependent effects for certain treatments. Plates were stored at 4°C. Fatty acid supplemented plates were covered with foil to prevent light oxidation. Plates were seeded with the different E. coli cultures (OD600 = 1.0) and dried for three days before use.
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