Imagescanner 3 apparatus

Manufactured by GE Healthcare

The ImageScanner III is a laboratory apparatus used for high-resolution scanning of various types of samples. It is capable of producing detailed digital images from physical specimens.

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Lab products found in correlation

En3hance spray, by PerkinElmer (2 mentions) Hybond xl, by GE Healthcare (2 mentions) Hoeffer uv crosslinker, by Hoefer (2 mentions)

Spelling variants of this product

ImageScanner III (177 citations) ImageScanner (54 citations)

2 protocols using imagescanner 3 apparatus

Quantifying mRNA Synthesis Dynamics

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5 × 10⁶ MM1.S cells overexpressing ACA11 or H929 cells transduced with ACA11 ASO2 knockdown cells were starved in methionine/cysteine‐free media for 30 minutes prior to labeling with ³H‐methyl‐methionine (Perkin Elmer, USA) for 30‐minutes. Following a 2‐hour chase in complete media with excess cold methionine, nuclear and cytoplasmic exacts were prepared as previously described.9 RNA was isolated using RNA‐solv (Omega BioTeck, Norcross, GA), separated on formaldehyde containing agarose gels, transferred to Hybond XL (GE Healthcare Life Sciences, Pittsburgh, PA), crosslinked using a Hoeffer UV Crosslinker (Hoefer, Inc, USA), and sprayed with EN3HANCE Spray (Perkin Elmer). The dried membrane was exposed to preflashed autoradiography film with an Intesifying screen at −80°C for 1‐2 weeks. Autoradiograms were scanned using an ImageScanner III apparatus (GE Healthcare Life Sciences) and densities were determined using ImageQuant V V.2005 software (GE Healthcare Life Sciecnes, Pittsburgh, PA). The data are a representative image of three independent experiments.

Oliveira V., Mahajan N., Bates M.L., Tripathi C., Kim K.Q., Zaher H.S., Maggi Jr L.B, & Tomasson M.H. (2019). The snoRNA target of t(4;14) in multiple myeloma regulates ribosome biogenesis. FASEB bioAdvances, 1(7), 404-414.

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Measuring mRNA Synthesis and Stability

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5×10⁶ MM1.S cells overexpressing ACA11 or H929 cells transduced with ACA11 ASO2 knockdown cells were starved in methionine/cysteine-free media for 30 minutes prior to labeling with ³H-methyl-methionine (Perkin Elmer) for 30-min. Following a 2-h chase in complete media with excess cold methionine, nuclear and cytoplasmic exacts were prepared as previously described (9 (link)). RNA was isolated using RNA-solv (Omega BioTeck, Norcross, GA, USA), separated on formaldehyde containing agarose gels, transferred to Hybond XL (GE Healthcare Life Sciences, Pittsburgh, PA), crosslinked using a Hoeffer UV Crosslinker (Hoefer, Inc. USA), and sprayed with EN3HANCE Spray (Perkin Elmer, USA). The dryed membrane was exposed to preflashed autoradiography film with an Intesifying screen at −80°C for 1 – 2 weeks. Autoradiograms were scanned using an ImageScanner III apparatus (GE Healthcare Life Sciences, Pittsburgh, PA) and densities were determined using ImageQuant V V.2005 software (GE Healthcare Life Sciecnes, Pittsburgh, PA). The data is a representative image of three independent experiments.

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