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2 protocols using hippuryl his leu acetate salt

1

Enzymatic Assays for Biological Activities

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2,2′‐Azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) ammonium salt (ABTS), casein, hemoglobin, hippuryl‐his‐leu acetate salt (HHL), lung acetone powder from rabbit, mushroom tyrosinase, potassium persulfate, and sodium L‐tartrate were purchased from Sigma‐Aldrich. 3,4‐Dihydroxy‐L‐phenylalanine (L‐DOPA) was purchased from Acros Organics. Hydrochloric acid (1 M) and sodium hydroxide (1 M) were purchased from Yakuri Pure Chemicals Co., Ltd.. Folin–Ciocalteu reagent and acetic acid were purchased from Junsei Chemical Co., Ltd. Sodium dodecyl sulfate (SDS) and glycine were purchased from Bio Basic, Inc. Soybean oil was purchased from Ottogi Co., Ltd. All other reagents were of analytical grade.
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2

Angiotensin-I-Converting Enzyme Assay

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Angiotensin-I-converting enzyme from rabbit lung, boric acid, BSA, Coomassie brilliant blue G-250, dglucose, dinitrosalicylic acid, Folin-Ciocalteu reagent, gallic acid, hippuryl-His-Leu acetate salt, 2-mercaptoEthanol, o-phthaldialdehyde, phenol, phenolphthalein, phosphoric acid, SDS, sodium tetraborate decahydrate, and trichloroacetic acid were purchased from Sigma Chemical Co. (St. Louis, MO). Ethanol, ethyl acetate, hydrochloric acid, mEthanol, sodium chloride, sodium hydroxide, sodium sulfite, and sulfuric acid were purchased from Merck (Darmstadt, Germany).
The M17 broth was purchased from Becton Dickinson and Company (Franklin Lakes, NJ). Nestle (Cabuyao, Laguna, Philippines) Carnation skim milk powder was purchased from the local market.
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