Ultra purified water

The LC/MS analyses were performed on a Shimadzu Nexera I LC/MS-8045 (Kyoto, Japan) UHPLC system. The separation was carried out on a Luna C18 reversed-phase column (Torrance, CA, USA). The mobile phase was a gradient made from methanol (Merck, Darmstadt, Germany) and ultrapurified water (Merck Millipore, Billerica, MA, USA). Formic acid was used as an organic modifier.
The detection was performed on a quadrupole rod mass spectrometer operated with electrospray ionization (ESI), both in negative and positive MRM (multiple reaction monitoring) ion mode. Chlorogenic acid, gallic acid, salicylic acid, catechin, apigenin, chrysin, hyperoside, luteolin-7-O-glucosid, naringenin, quercetin and rutoside were used as standards. A quantity of 1 mL was injected from each standard at each concentration. The identification was performed by comparison of the MS. The identification and quantification were made based on the main transition from the MS spectra of the substance.

Hydrogenated palm oil (Condea Chemie, Hamburg, Germany) was a gift from the Malaysian Palm Oil Board (MPOB) and olive oil was purchased from Basso Fegele and Figli Srl (San Michele di Serino, Italy). Others ingredients included were polysorbate 80 (Thermo Fisher Scientific, Waltham, MA, USA) Ultrapurified water (Merck Millipore, Billerica, MA, USA) and Lipoid S100 (lecithin) (Lipoid GmbH, Ludwigshafen, Switzerland).
Thimerosal, sorbitol, bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), Tamoxifen free base, 4',6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), ribonuclease A (Rnase A), thiazolyl blue tetrazolium bromide (MTT), Harris’s haematoxylin and eosin (H&E), ketamine hydrochloride, xylazine hydrochloride, horse serum, epidermal growth factor (EGF), hydrocortisone and insulin were purchased from Sigma-Aldrich (St Loius, MO, USA). Recombinant human erythropoietin was purchased from Peprotech (Rocky Hill, NJ, USA), paraformaldehyde (Acros Organics, USA), normal saline (0.9% NaCl), and 10% buffered formalin and Triton X–100 from Thermo Fisher Scientific (United States).
Rat mammary gland tumor cell (LA7) and non-tumorigenic breast (MCF-10A) cells were purchased from the American Type and Culture Collection (ATCC, Manassas, VA, USA).

Ethanol (HPLC grade; Tedia^®, Fairfield, OH, USA) was used in the extraction procedure, while methanol, formic acid (LC-MS grade; Merck^®, Darmstadt, Germany), and ultrapurified water (Millipore^®, Burlington, MA, USA) were used as mobile phase components.