miR-195-5p mimic and the negative control miRNA (miRNA-NC) were obtained from Guangzhou RiboBio Co., Ltd. The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd. The sequences were as follows: miR-195-5p mimic sense, 5'-UAGCAGCACAGAAAUAUUGGC-3'; miR-195-5p mimic antisense, 5'-CAAUAUUUCUGUGCUGCUAUU-3'; mimics negative control (miR-NC) sense, 5'-UUCUCCGAACGUGUCACGUTT-3'; miR-NC antisense, 5'-ACGUGACACGUUCGGAGAATT-3'. Cells were plated into 6-well plates (1x106 cells per well), and transfection was performed when cells at the logarithmic growth phase reached 80% confluence. According to the product instructions, AMC-HN-8 cells were transfected with miR-195-5p mimic (50 nM), miR-NC (50 nM), pcDNA-E2F3 (100 nM) and pcDNA-NC (100 nM) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). The transfection efficiencies were assessed by RT-qPCR at 48 h after transfection.
Pcdna e2f3
Manufactured by GenePharma
Sourced in China
PcDNA-E2F3 is a plasmid vector that contains the E2F3 gene. E2F3 is a member of the E2F family of transcription factors, which play a key role in the regulation of cell cycle progression. The PcDNA-E2F3 vector can be used for the expression of the E2F3 gene in various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (5 mentions)
Mir 194 inhibitor, by GenePharma (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Ges 1, by American Type Culture Collection (4 mentions)
Pcdna nc, by Thermo Fisher Scientific (1 mentions)
Mir 195 5p mimic, by GenePharma (1 mentions)
Mir 195 5p mimic, by RiboBio (1 mentions)
Pcdna nc, by GenePharma (1 mentions)
Mirna nc, by RiboBio (1 mentions)
Mir nc, by GenePharma (1 mentions)
5 protocols using pcdna e2f3
1
miR-195-5p Regulation of E2F3 in AMC-HN-8 Cells
2
Gastric Cancer Cell Line Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The gastric cancer cell lines (SGC-7901, SUN-16, MKN-45, and AGS cells) and the human gastric epithelial cell line GES-1 (American Type Culture Collection, USA) were used in this study. All cells cultured respectively in Dulbecco's Modi ed Eagle Medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin (Hyclone, USA) and incubated in a 5% carbon oxide (CO 2 ) incubator at 37°C. For cell transfection, cells in logarithmic growth phase were transfected with corresponding constructs when the con uence was up to 80% following the instructions of Lipofectamine2000 (Invitrogen, USA).
The culture medium was replaced 6 hours later. H19-small interfering RNA (si-H19), miR-194 mimic, miR-194 inhibitor, pcDNA-E2F3, and negative control (NC) were constructed by Gene Pharma (Shanghai, China).
The culture medium was replaced 6 hours later. H19-small interfering RNA (si-H19), miR-194 mimic, miR-194 inhibitor, pcDNA-E2F3, and negative control (NC) were constructed by Gene Pharma (Shanghai, China).
3
Gastric Cancer Cell Transfection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The gastric cancer cell lines (SGC-7901, SUN-16, MKN-45, and AGS cells) and the human gastric epithelial cell line GES-1 (American Type Culture Collection, USA) were used in this study. All cells cultured respectively in Dulbecco's Modi ed Eagle Medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin (Hyclone, USA) and incubated in a 5% carbon oxide (CO 2 ) incubator at 37°C. For cell transfection, cells in the logarithmic growth phase were transfected with corresponding constructs when the con uence was up to 80% following the instructions of Lipofectamine2000 (Invitrogen, USA). The culture medium was replaced 6 hours later. H19-small interfering RNA (si-H19), miR-194 mimic, miR-194 inhibitor, pcDNA-E2F3, and negative control (NC) were constructed by Gene Pharma (Shanghai, China).
4
Gastric Cancer Cell Line Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The gastric cancer cell lines (SGC-7901, SUN-16, MKN-45, and AGS cells) and the human gastric epithelial cell line GES-1 (American Type Culture Collection, USA) were used in this study. All cells cultured respectively in Dulbecco's Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin (Hyclone, USA) and incubated in a 5% carbon oxide (CO 2 ) incubator at 37°C.
For cell transfection, cells in the logarithmic growth phase were transfected with corresponding constructs when the confluence was up to 80% following the instructions of Lipofectamine2000 (Invitrogen, USA). The culture medium was replaced 6 hours later. H19-small interfering RNA (si-H19), miR-194 mimic, miR-194 inhibitor, pcDNA-E2F3, and negative control (NC) were constructed by Gene Pharma (Shanghai, China).
For cell transfection, cells in the logarithmic growth phase were transfected with corresponding constructs when the confluence was up to 80% following the instructions of Lipofectamine2000 (Invitrogen, USA). The culture medium was replaced 6 hours later. H19-small interfering RNA (si-H19), miR-194 mimic, miR-194 inhibitor, pcDNA-E2F3, and negative control (NC) were constructed by Gene Pharma (Shanghai, China).
5
Gastric Cancer Cell Transfection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The gastric cancer cell lines (SGC-7901, SUN-16, MKN-45, and AGS cells) and the human gastric epithelial cell line GES-1 (American Type Culture Collection, USA) were used in this study. All cells cultured respectively in Dulbecco's Modi ed Eagle Medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin (Hyclone, USA) and incubated in a 5% carbon oxide (CO 2 ) incubator at 37°C. For cell transfection, cells in the logarithmic growth phase were transfected with corresponding constructs when the con uence was up to 80% following the instructions of Lipofectamine2000 (Invitrogen, USA). The culture medium was replaced 6 hours later. H19-small interfering RNA (si-H19), miR-194 mimic, miR-194 inhibitor, pcDNA-E2F3, and negative control (NC) were constructed by Gene Pharma (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!