Lymph nodes and spleens were harvested from vaccinated mice. These organs were then processed into a single cell suspension after being passed through a 40 μM strainer, lysed with ammonium-chloride-potassium (ACK) solution, and washed in Hank’s balanced salt solution (HBSS) containing 3% FBS before staining and fixation in 1% paraformaldehyde. Immune cell populations were identified by flow cytometry using an LSRII (BD Biosciences, San Jose, CA) and analyzed by FlowJo software (Tree Star, Ashland, OR). Lymph nodes or splenocytes were stained with anti-mouse CD3-FITC and anti-mouse CD44-APC purchased from eBioscience (San Diego, CA). Additionally, cells were stained for anti-mouse CD4-APC-Cy7, anti-mouse CD62L PeCy7, anti-mouse CD8-Pacific Blue, anti-mouse CD95-PE, anti-mouse CD19-PECy7, and anti-mouse GL-7 Pacific Blue (Biolegend, San Diego, CA).
Anti mouse cd95 pe
Manufactured by BioLegend
Sourced in United States
Anti-mouse CD95-PE is a fluorescently-labeled antibody that specifically binds to the mouse CD95 (Fas) cell surface receptor. CD95 is a member of the tumor necrosis factor receptor superfamily and is involved in the regulation of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cd3 fitc, by Thermo Fisher Scientific (1 mentions)
Pe cy7 anti mouse cd19, by BioLegend (1 mentions)
Pe cy7 anti mouse cd62l, by BioLegend (1 mentions)
Anti mouse cd4 apc cy7, by BioLegend (1 mentions)
Pe anti mouse cd45, by BioLegend (1 mentions)
Pe anti mouse sca 1, by BioLegend (1 mentions)
Pe anti mouse cd44, by BioLegend (1 mentions)
Anti fasl af647 antibody mlf4 clone, by Bio-Rad (1 mentions)
Cytoflex flow cytometer, by Beckman Coulter (1 mentions)
Modfit lttm software, by Verity Software House (1 mentions)
2 protocols using anti mouse cd95 pe
1
Lymphocyte Profiling in Vaccinated Mice
2
Flow Cytometric Profiling of Murine MSCs
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For flow cytometric analysis, MSC were stained with the following antibodies: anti-mouse Sca1PE, anti-mouse CD73PE/Cy7, anti-mouse CD117APC (c-kit), anti-mouse CD45PE, anti-mouse CD44PE, anti-mouse CD95PE (Biolegend, San Diego, CA, USA), goat anti-mouse CD105 and the secondary rabbit anti-goatFITC (Invitrogen, Carlsbad, CA, USA). The antibody mix that was used to label the splenocytes contained anti-mouse CD45PE, anti-mouse CD4BV784 and anti-mouse CD8aAPCfire (Biolegend, San Diego, CA, USA). FasL was detected using anti-FasLAF647 antibody (MLF4 clone, Bio-Rad, CA, USA). For all the antibodies, we used the corresponding isotypes from the same sources. Samples were analyzed using a Cytoflex flow cytometer (Beckman Coulter, Indianapolis, IN, USA) and CytExpert software. Determination of the proliferation index of the splenocytes was done based on the carboxyfluorescein succinimidyl ester (CFSE) readings that were processed using ModFit LTTM software (Verity Software House, Topsham, ME, USA). For the CFSE analysis, the unstimulated splenocytes were used as the parent (basal fluorescence).
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