After transfection, 100 μl cells (1 × 103) were plated into 96-well plates and cultured for 1 h. Subsequently, cells were mixed with 10 μl CCK8 regent (Yeasen) for 2 h. The optical density (OD) was detected at 450 nm through an automatic microplate reader (Molecular Devices, Suzhou, China).
Cck8 regent
Manufactured by Yeasen
Sourced in China
The CCK8 reagent is a colorimetric assay used for the determination of cell viability and cell proliferation. It contains a water-soluble tetrazolium salt that is reduced by living cells, producing a colored formazan dye. The amount of formazan dye produced is directly proportional to the number of viable cells in the sample.
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Lab products found in correlation
6 protocols using cck8 regent
1
Cell Viability Assay by CCK8
2
Cell Viability Assay by CCK8
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After transfection, 100 μl cells (1 × 103) were plated into 96-well plates and cultured for 1 h. Subsequently, cells were mixed with 10 μl CCK8 regent (Yeasen) for 2 h. The optical density (OD) was detected at 450 nm through an automatic microplate reader (Molecular Devices, Suzhou, China).
3
Cell Proliferation Assay Using CCK8
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Cell proliferation was determined by CCK8 assay. Briefly, ANP32E silenced or over-expressed and the control cells were seeded in triplicate into 96-well plates at the density of 2000 cells per well. The cells were cultured for 4 days. 1, 2, 3 and 4 days later, 10% the CCK8 regent (YEASEN) was added into each well and the plates were maintained at 37 °C. 3 h later, the OD value at 450 nm was measured on the micro-plate machine. The cell viability was normalized to the OD value of the first day.
4
Cell Proliferation Measurement by CCK8 Assay
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Cell proliferation was determined by CCK8 assay. Brie y, ANP32E silenced or over-expressed and its control cells were seeded into 96-well plates at the density of 2000 cells per well. The cells were cultured for 4 days. 1, 2, 3 and 4 days later, 10% the CCK8 regent (YEASEN) was added into each well and the plates were maintained at 37 o C. 3 hours later, the OD value at 450 nm was measured on the micro-plate machine. The cell viability was normalized to the OD value of the rst day.
5
Cell Proliferation Evaluation by CCK8 Assay
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Cell proliferation was determined by CCK8 assay. Briefly, ANP32E silenced or over-expressed and its control cells were seeded into 96-well plates at the density of 2000 cells per well. The cells were cultured for 4 days. 1, 2, 3 and 4 days later, 10% the CCK8 regent (YEASEN) was added into each well and the plates were maintained at 37 o C. 3 hours later, the OD value at 450 nm was measured on the micro-plate machine. The cell viability was normalized to the OD value of the first day.
6
Cell Proliferation Evaluation by CCK8 Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was determined by CCK8 assay. Briefly, ANP32E silenced or over-expressed and its control cells were seeded into 96-well plates at the density of 2000 cells per well. The cells were cultured for 4 days. 1, 2, 3 and 4 days later, 10% the CCK8 regent (YEASEN) was added into each well and the plates were maintained at 37 o C. 3 hours later, the OD value at 450 nm was measured on the micro-plate machine. The cell viability was normalized to the OD value of the first day.
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