α tomm20

Manufactured by Santa Cruz Biotechnology

Sourced in United States

α-TOMM20 is a primary antibody that targets the Translocase of Outer Mitochondrial Membrane 20 (TOMM20) protein. TOMM20 is a key component of the protein import machinery in the mitochondrial outer membrane and plays a crucial role in the transport of proteins into the mitochondria.

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Lab products found in correlation

α flag, by Merck Group (2 mentions) Slowfade gold antifade reagent, by Thermo Fisher Scientific (1 mentions) α atp5a, by Abcam (1 mentions) α pcna, by Santa Cruz Biotechnology (1 mentions) α qil1, by Merck Group (1 mentions) α cytochrome c, by Cell Signaling Technology (1 mentions) α pmp70, by Abcam (1 mentions) Mouse monoclonal α his, by Merck Group (1 mentions)

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TOMM20 (25 citations)

4 protocols using α tomm20

Immunolabeling of Mitochondria and Cytoskeleton

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Cells grown on 15-mm glass coverslips or 384-well clear bottom plates were fixed with PBS, 4% PFA, and 4% Sucrose for 10 min, permeabilized with PBS 0.1% Triton X-100 for 3 min, blocked with PBS 1% BSA for 30 min, and incubated with α-TOMM20 (1:100; Santa Cruz) and α-HA (1:200; Covance) in PBS 1% BSA for one hour at room temperature. After extensive washing, fixed cells were incubated with Alexa488-chicken anti-rabbit and Alexa594-goat anti-mouse (1:10000) secondary antibodies for 1 hr at room temperature. For the in vivo analysis, Drosophila muscle tissue was dissected in PBS, fixed for 30 min in 4% paraformaldehyde, washed, permeabilized for 2 hr in 0.5% Triton X-100 in PBS, and blocked with normal goat serum in 0.1% Tween in PBS (PBST). α-ATP5A primary antibody (1:300; Abcam) was diluted in blocking buffer and incubated overnight at 4°C. After extensive washing with PBST, tissues were incubated with the secondary antibody (Alexa488-chicken anti-rabbit, 1:10000) and Phalloidin (1:10000, Invitrogen) for 2 hr. Tissues were washed and mounted in SlowFade Gold antifade reagent (Invitrogen). Images were acquired with a Nikon confocal microscope with a 100× oil objective.

Guarani V., McNeill E.M., Paulo J.A., Huttlin E.L., Fröhlich F., Gygi S.P., Van Vactor D, & Harper J.W. (2015). QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. eLife, 4, e06265.

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Mitochondrial Protein Detection Protocol

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Antibodies used in this work include: α-QIL1 (Sigma SAB1102836), α-MINOS1 (Aviva, San Diego, USA, ARP44801-P050), α-CHCHD3 (Aviva ARP57040-P050), α-CHCHD6 (Proteintech, Chicago, USA, 20639-1-AP), α-IMMT (Abcam, Cambridge, UK, ab110329), α-TIMM23 (BD-Biosciences, Franklin Lakes, USA, 611222), α-SAMM50 (Proteintech 20824-1-AP), α-APOOL (Aviva OAAF03292), α-PCNA (Santa Cruz, Dallas, USA, sc-56), α-HSP90 (Epitomics, Burlingame, USA, 3363-1), α-TOMM70A (Epitomics T1677),α-Flag (Sigma SLB6631), α-HA (Covance, Princeton, USA, D13CF00834), α-ATP5A (Abcam ab14748), α-DLD (Santa Cruz sc-271569), α-TMEM11 (Proteintech 16564-1-AP), α-Cytochrome C (Cell Signaling, Danvers, USA, 4272S), α-TOMM20 (Santa Cruz sc-11415), Phalloidin (Invitrogen A22287), and α-APOO (Novus Bio, Littleton, USA, NBP1-28870).

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Antibody Sourcing for Cell Biology

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Rabbit polyclonal α-PMP70 was purchased from Abcam. Rabbit polyclonal α-NBR1 was a gift from I. Dikic (Goethe Univerity, Frankfurt, Germany). Human polyclonal α-P0 was a gift from A. Palazzo (University of Toronto, Toronto, Canada). Mouse monoclonal α-TOMM20 was purchased from Santa Cruz Biotechnology, Inc. Mouse monoclonal α-Mfn2 was purchased from Cedarlane. Rabbit monoclonal α-calnexin was a gift from D. Williams (University of Toronto, Toronto, Canada). Mouse monoclonal α-HA was purchased from Covance. Rabbit polyclonal α-Atg12 was purchased from Cell Signaling Technology. Rabbit polyclonal α-PEX14 was purchased from Millipore. Rabbit polyclonal α-PEX2 was generated from peptide by GenScript. Rabbit polyclonal α-PEX2 was purchased from Abcam. Rabbit polyclonal α-PEX5 was generated by GenScript. Rabbit polyclonal α-catalase was purchased from EMD Millipore. Rabbit monoclonal α-4E-BP was a gift from J. Brumell (University of Toronto, Toronto, Canada). Mouse monoclonal α-FLAG was purchased from Sigma-Aldrich.
HRP-conjugated goat polyclonal α-mouse secondary and HRP-conjugated goat polyclonal α-rabbit secondary was purchased from Cedarlane. HRP-conjugated mouse monoclonal α-GAPDH was purchased from Abcam. Fluorescent goat polyclonal α-rabbit Alexa Fluor 568 secondary antibody and goat polyclonal α-mouse Alexa Fluor 488 secondary antibody were purchased from Invitrogen.

Sargent G., van Zutphen T., Shatseva T., Zhang L., Di Giovanni V., Bandsma R, & Kim P.K. (2016). PEX2 is the E3 ubiquitin ligase required for pexophagy during starvation. The Journal of Cell Biology, 214(6), 677-690.

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Immunoblotting of Mitochondrial Proteins

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mouse monoclonal α-CYCS (BD Pharmingen, Franklin Lakes, USA, Cat # 556432), goat polyclonal α-ENO (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-7455), mouse monoclonal α-His (Sigma-Aldrich, Saint Louis, USA, Cat # SAB1305538), mouse monoclonal α-LRPPRC (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-166178), rabbit polyclonal α-OPA1 (Proteintech Group, Rosemont, USA, Cat # 27733-1-AP), rabbit polyclonal α-PNPase (Abcam, Cambridge, UK, Cat # ab96176), mouse monoclonal α-Ro60 (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-100844), rabbit polyclonal α-Ro60 (Sigma-Aldrich, Saint Louis, USA, Cat # HPA002835), rabbit polyclonal α-TOMM20 (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-11415), rabbit polyclonal α-uL4m (Proteintech Group, Rosemont, USA, Cat # 27484-1-AP), polyclonal sheep HRP-conjugated α-mouse (GE Healthcare, Chicago, USA, Cat # NXA931), polyclonal donkey HRP-conjugated α-rabbit (GE Healthcare, Chicago, USA, Cat # NA934V).

Jeandard D., Smirnova A., Fasemore A.M., Coudray L., Entelis N., Förstner K.U., Tarassov I, & Smirnov A. (2022). CoLoC-seq probes the global topology of organelle transcriptomes. Nucleic Acids Research, 51(3), e16.

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