Amphotericin B, as the active pharmaceutical ingredient, was bought from Bio Basic Inc. (Toronto, ON, Canada). Intravenous Amphotericin B was supplied by Biolab (Samutprakarn, Thailand). Polyethylene glycol 400 (PEG) was purchased from Nam Siang Co. Ltd. (Bangkok, Thailand). Branched polyethylenimine (PEI), hyaluronic acid, mucin from porcine stomach type II, hydrocortisone, insulin from bovine pancreas, and fluorescein isothiocyanate were ordered from Sigma-Aldrich (St. Louis, MO, USA). Poloxamer 407 (Pluronic® F127) was acquired from BASF (Florham Park, NJ, USA). Muller-Hinton agar and Sabouraud Dextrose agar were purchased from HIMEDIA (Mumbai, India). Roswell Park Memorial Institute (RPMI) 1640 medium, keratinocyte serum free medium (K-SFM), bovine pituitary extract (BPE), epithelial growth factor (EGF), and Penicillin/ Streptomycin solution were ordered from Gibco (New York, NY, USA). Thiazolyl blue tetrazolium bromide was acquired from Amresco® (Solon, OH, USA). Crystal violet was supplied by Riedel-de Haën (Munich, Germany). All other reagents were of analytical grade or higher.
Amphotericin b
Manufactured by Bio Basic
Sourced in Canada
Amphotericin B is a broad-spectrum antifungal agent used in laboratory settings. It is a polyene macrolide compound derived from the bacterium Streptomyces nodosus. Amphotericin B is primarily used to inhibit the growth of a variety of fungi and yeasts.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Insulin from bovine pancreas, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide, by Avantor (1 mentions)
Mucin from porcine stomach type 2, by Merck Group (1 mentions)
Keratinocyte serum free medium, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Muller hinton agar, by HiMedia (1 mentions)
Sabouraud dextrose agar, by HiMedia (1 mentions)
Poloxamer 407 pluronic f 127, by BASF (1 mentions)
Hexane, by Loba Chemie (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Neutral red solution, by Merck Group (1 mentions)
Tetracycline hydrochloride, by Merck Group (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Fluorescein sodium salt, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Thermo Fisher Scientific (1 mentions)
3 protocols using amphotericin b
1
Amphotericin B Nanoformulation for Topical Delivery
2
Antifungal Susceptibility Testing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Broth dilution antifungal susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) M38-A2(25 ). In total, four types of antifungal agents were tested, including amphotericin B (CAS: 1397-89-3; Bio Basic, Inc.), caspofungin acetate (CAS: 179463-17-3; Beijing Jin Ming Biotechnology Co., Ltd.), fluconazole (CAS: 86386-73-4; Rhawn Reagent) and fluorocytosine (CAS: 2022-85-7; Rhawn Reagent). Different reagent grades were tested in the following concentrations according the manufacturers' instructions: amphotericin B 0.03-16 µg/ml, caspofungin 0.03-16 µg/ml, fluconazole 0.12-64 µg/ml, fluorocytosine 0.12-64 µg/ml. The susceptibility of this fungus to each drug was determined.
3
Comprehensive Chemical Inventory for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following chemicals were used in the study: amphotericin B from Bio Basic (Canada), Roswell Park Memorial Institute-1640 medium from Biowest (USA), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3-(N-morpholino)propanesulfonic acid from Calbiochem (Germany), chloramphenicol from Duchefa Biochemie (The Netherlands), foetal bovine serum, penicillin--streptomycin solution, trypan blue, and trypsin-EDTA solution from Gibco (USA), Mueller--Hinton agar and Mueller-Hinton broth from HiMedia (India), potato dextrose agar from Laboratorios Conda (Spain), ethyl acetate (AR grade), ferric(III) chloride hexahydrate, Sabouraud dextrose agar, Dragendorff's reagent, and sodium acetate from Merck (Germany), chloroquine diphosphate salt from MP Biomedicals (USA), 2,4,6-tripyridyl-s-triazine from Nacalai Tesque (Japan), Dulbecco's modified Eagle's medium (DMEM), Dulbecco's phosphate buffered saline, fluorescein sodium salt, gelatine, neutral red solution, p-iodonitrotetrazolium chloride, sodium bicarbonate, and tetracycline hydrochloride from Sigma-Aldrich (USA), ferrous sulphate heptahydrate from Loba Chemie (India), hexane, 95 % ethanol, chloroform, methanol (all of AR grade), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) from Thermo Fisher Scientific (USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!