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The SW1990 is a cell line product from China Infrastructure of Cell Line Resources. It is a pancreatic ductal adenocarcinoma cell line derived from a human patient. The core function of the SW1990 is to serve as a research tool for the study of pancreatic cancer.

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3 protocols using sw1990

1

Culturing and Maintaining Pancreatic Cancer Cell Lines

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Human pancreatic cancer cell line PANC-1, SW1990, and BxPC-3 were obtained from China Infrastructure of Cell Line Resources (Beijing, China). Capan-2 and MIA PaCa-2 were purchased from Obio Technology (Shanghai, China) Corp., Ltd. SW1990, PANC-1 and MIA PaCa-2 were cultured in DMEM, Capan-2 was maintained in McCoy’s 5A and BxPC-3 was cultured in RMPI 1640, all supplemented with 10% fetal bovine serum, 100 U/ml of penicillin and 100 μg/ml of streptomycin. All of the cells were incubated at 37 ℃ in a humidified atmosphere of 5% CO2. The gemcitabine (Gemzar) drug was purchased from Eli Lilly and Company (Indianapolis, Indiana, USA).
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2

Culturing PC Cell Lines in Mice

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The PC cell lines Panc1, CFPAC1, Bxpc3, SW1990, AsPC1, and HPDE6-C7 were purchased from China Infrastructure of Cell Line Resources (Beijing, China) and cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Cleveland, OH, USA) supplemented with 10% fetal bovine serum at 37°C in an atmosphere containing 5% CO2. BALB/c male nude mice were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The mice experiments were approved by the Animal Experimental Ethics Committee of PLA General Hospital and conducted in the animal facility of PLA General Hospital according to the animal experimental ethics committee guidelines of PLA General Hospital.
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3

Cultivation of Pancreatic Cell Lines

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Pancreatic cancer cell lines AsPC-1, BxPC-3, CFPAC-1, PANC-1, and SW1990 and human pancreatic duct epithelial cell line HPDE6-C7 were purchased from China Infrastructure of Cell Line Resources (Beijing, People’s Republic of China) and cultured in Dulbecco’s Modified Eagle’s Medium (Hyclone Co., Logan, UT, USA) and supplemented with 10% FBS (Hyclone Co.) at 37°C in a humidified 5% CO2 air incubator.
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