Anti sr b1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-SR-B1 is a laboratory product developed by Santa Cruz Biotechnology. It is a specific antibody that targets the scavenger receptor class B type 1 (SR-B1) protein. The core function of Anti-SR-B1 is to enable the detection and study of the SR-B1 protein in various experimental settings.

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Lab products found in correlation

Ecl detection kit, by Cytiva (1 mentions) Anti ki67, by Santa Cruz Biotechnology (1 mentions) Anti lox 1, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin, by Lab Vision (1 mentions) Hybond ecl nitrocellulose membrane, by GE Healthcare (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Anti occludin, by Thermo Fisher Scientific (1 mentions) Anti claudin 1, by Thermo Fisher Scientific (1 mentions) Hybond, by Cytiva (1 mentions)

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SR-B1 (5 citations)

2 protocols using anti sr b1

Protein Expression Analysis in Keratinocytes

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Total cell lysates were extracted from keratinocytes. Proteins were separated by SDS-PAGE and transferred to a PVDF membrane. The membranes were probed using anti-Ki67 (Santa Cruz, CA, USA), anti-LOX-1 (Santa Cruz, Dallas, TX, USA), anti-scavenger receptor A (SRA; Santa Cruz, CA, USA), anti-SR-B1 (Santa Cruz, Dallas, TX, USA), and anti-IL23 (Novusbio, Littleton, CO, USA) antibodies. Mouse anti-β-actin (Labvision/NeoMarkers, Fremont, CA, USA) antibody was used as a loading control. The proteins were visualized using an enhanced chemiluminescence (ECL) detection kit (Amersham Biosciences, Waltham, MA, USA) and quantified using a densitometry.

Shih C.M., Huang C.Y., Wang K.H., Huang C.Y., Wei P.L., Chang Y.J., Hsieh C.K., Liu K.T, & Lee A.W. (2018). Oxidized Low-Density Lipoprotein-Deteriorated Psoriasis Is Associated with the Upregulation of Lox-1 Receptor and Il-23 Expression In Vivo and In Vitro. International Journal of Molecular Sciences, 19(9), 2610.

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Western Blot Analysis of Membrane Proteins

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Cells were lysed with RIPA buffer. Protein content of cell lysates was determined using the BCA protein assay kit (Pierce). The proteins were precipitated, separated by SDS-PAGE and transferred to Hybond-ECL nitrocellulose membranes (GE Healthcare). Proteins were detected with specific antibodies diluted 1:1,000 (polyclonal anti-SR-B1 (Santa Cruz, ref sc-67098), polyclonal anti-Occludin (Invitrogen), polyclonal anti-Claudin 1 (Invitrogen), polyclonal anti-NPC1L1 (Cell Signaling), polyclonal anti-ApoE (AB947 Millipore) and monoclonal anti-actin (C4, Millipore) and secondary antibodies conjugated to peroxidase (Sigma-Aldrich). The immune complexes were visualized by enhanced chemiluminescence detection (ECL; GE Healthcare) as recommended by the manufacturer.

Fournier C., Hoffmann T.W., Morel V., Descamps V., Dubuisson J., Brochot E., Francois C., Duverlie G., Castelain S, & Helle F. (2018). Claudin-1, miR-122 and apolipoprotein E transductions improve the permissivity of SNU-182, SNU-398 and SNU-449 hepatoma cells to hepatitis C virus. Journal of viral hepatitis, 25(1).

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