Dl ap 5 sodium salt

Dyes and drugs were purchased from the following suppliers: (RS)-AMPA hydrobromide (AMPA), Kainate (KA), GYKI 52466 hydrochloride (GYKI), DL-threo-β-Benzyloxyaspartic acid (TBOA), NBQX disodium salt (NBQX), DL-AP5 sodium salt (APV), dynamin inhibitory peptide (QVPSRPNRAP), dynamin inhibitory peptide myristoylated (scrambled), dynamin inhibitory peptide myristoylated, cyclothiazide, mecamylamine, dhβe, and Dynasore (Tocris, Ellisville, MO, USA); N-Methyl-D-Aspartate (NMDA), tetrodotoxyn (TTX), Strychnine, Bicuculline and L-glutamate, atropine (Sigma, St Louis, MO); Fluoro-Gold (Fluorochrome, Inc., Denver, CO, USA); Lucifer Yellow (LY), Acridine Orange 10-Nonyl (AO), 6-methoxy-N-ethylquinolinium iodide (MEQ), and Florescien-5-isothiocyan (FITC) were from Invitrogen. Dynasore was dissolved in DMSO. When bath applied to the cord, the concentration of DMSO was less than 0.1%. All other drugs were dissolved in artificial cerebrospinal fluid (aCSF).

D‐luciferin was purchased from Gold Biotechnology (St. Louis, MO), prepared as a 30 mg/ml stock solution in phosphate‐buffered saline (PBS), and stored at −20°C. Peptides were synthesized from Zhejiang Ontores Biotechnologies Co. (Yuhang District, Hangzhou, Zhejiang, China). Peptides were dissolved in either 100% Dimethyl sulfoxide (DMSO) or HBS‐P⁺ buffer (100 mM hydroxyethylpiperazine ethane sulfonic acid (HEPES), 150 mM NaCl, 0.005% (v/v) P20, pH 7.4; GE Healthcare Bio‐Sciences, Pittsburgh, PA) to prepare 100 mM stock solutions and stored at −20°. For slice electrophysiology the following glutamatergic and GABAergic synaptic transmission blockers were used; 2,3‐dihydroxy‐6‐nitro‐7‐sulfamoylbenzo (f) quinoxaline (NBQX) disodium salt at a final concentration of 20 µM (Tocris, MN) of prepared from stock solution (100 mM in H₂O); (+)‐Bicuculline 20 µM (Sigma‐Aldrich, MO) of stock solution (100 mM in DSMO); and D and L forms of 2‐amino‐5‐phosphonovalerate and 2‐amino‐4‐phosphonobutyrate (DL‐AP5) sodium salt 100 µM (Tocris) of stock solution in (H₂O 100 mM) were stored at −20° and 4°C respectively.

DL-TBOA, DL-AP-5 sodium salt, MK-801 maleate, ketamine hydrochloride, NBQX disodium salt, were purchased from Tocris Bioscience (Bristol, UK).