5 fluouracil

Manufactured by Merck Group

5-fluorouracil is a lab equipment product used as a chemical compound. It serves as a pyrimidine analog, a class of organic compounds that are structural analogs of pyrimidine. The core function of 5-fluorouracil is to act as a building block in various biochemical processes.

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Lab products found in correlation

Puromycin, by Merck Group (2 mentions) Pd98059, by Merck Group (2 mentions) Mk 2206, by Selleck Chemicals (2 mentions) Spermine, by Merck Group (1 mentions) Linolenic acid, by Merck Group (1 mentions) Curcumin, by Merck Group (1 mentions) Metformin, by Merck Group (1 mentions) Epigallocatechin gallate (egcg), by Merck Group (1 mentions) Paclitaxel, by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions) Silibinin, by Merck Group (1 mentions)

Spelling variants of this product

5-fluouracil (5-FU) (2 citations)

2 protocols using 5 fluouracil

Preclinical Evaluation of Anti-Cancer Agents

Cited 1 time

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Puromycin, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DCA, linolenic acid (LA), spermine, cisplatin (CDDP), 5-fluouracil (5-FU), Paclitaxel (TAXOL), curcumin, epigallocatechin gallate (EGCG), silibinin (INN), metformin, ERK1/2 inhibitor PD98059 and glutaminase inhibitor 6-diazo-5-oxo-L-norlucine (DON) were purchased from Sigma. PKB/Akt inhibitor MK-2206 was obtained from Selleckchem. HNSCC cell lines including SAS and HSC3 (tongue cancer), FaDu (hypopharyngeal cancer) and OECM-1 (Oral squamous cell carcinoma, OSCC) were obtained elsewhere [23] (link). Animal procedures were approved in accordance with the Institutional Animal Care and Use Committee (IACUC), National Yang-Ming University (NYMU) [23] (link), [25] (link). For xenografic tumor growth assay, cells were subcutaneously injected at the back of nude mice from National Laboratory Animal Centre and tumor size/mass was recorded. Clinical human HNSCC tissues (Supplementary Table 1) were obtained under approval of the Institutional Review Boards (IRB) of MacKay Memorial Hospital (IRB approval number: 12MMHIS178) and National Taiwan University Hospital (IRB approval number: 203106005RINC), Taiwan.

Chen T.Y., Hsieh Y.T., Huang J.M., Liu C.J., Chuang L.T., Huang P.C., Kuo T.Y., Chia H.Y., Chou C.Y., Chang C.W., Chen Y.F., Chen H.M., Lo J.F, & Li W.C. (2019). Determination of Pyruvate Metabolic Fates Modulates Head and Neck Tumorigenesis. Neoplasia (New York, N.Y.), 21(7), 641-652.

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Xenograft Tumor Growth Assay of HNC

Cited 1 time

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Puromycin, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), CDDP, 5-fluouracil (5-FU), and Extracellular signal-regulated kinase 1/2 (ERK1/2) inhibitor PD98059 were purchased from Sigma-Aldrich. Protein kinase B (PKB/Akt) inhibitor MK-2206 was obtained from Selleckchem. The source and culture conditions of HNC cells (all are Human PapillomaVirus negative (HPV⁻)) from different origins as well as in HNC bearing xenografic tumour growth assay [under the approval of Institutional Animal Care and Use Committee (IACUC), National Yang Ming Chiao Tung University (NYCU) were described elsewhere [26 (link), 27 (link)]. In brief, the same kind of 10⁶ control (shLuc) and TFAM silencing HNC cells were transplanted in opposite side of flank in the same male nude mouse (5–7 weeks old CAnN.Cg-Foxn1nu/CrlNarl from Academia Sinica N = 9–10). The tumour size was followed every week and at the 4th week post transplantation, tumours were excised and weighted. Clinical human HNC tumours and its adjacent normal tissues were obtained from the Department of Oral Maxillofacial Surgery, NYCU Hospital under approval of the Institutional Review Boards (IRB) of NYCU Hospital (IRB#: NYMUH 2018B003) and were preserved in RNAlater immediately after surgical resection for qRT-PCR analysis (Table S1).

Hsieh Y.T., Tu H.F., Yang M.H., Chen Y.F., Lan X.Y., Huang C.L., Chen H.M, & Li W.C. (2021). Mitochondrial genome and its regulator TFAM modulates head and neck tumourigenesis through intracellular metabolic reprogramming and activation of oncogenic effectors. Cell Death & Disease, 12(11), 961.

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