Intestinal organoids were obtained as previously described (Sato et al., 2009 (link)). Organoids were treated as follows: mouse recombinant IL-11 (R&D; 418-ML) at 50 ng/ml; mouse recombinant IL-22 (R&D; 582-ML) at 50 ng/ml; IFN-γ (eBiosciences 34-8311-85) at 50 ng/ml for 24 h followed by 16 h of BrdU staining (1/100 dilution of the BrdU labeling reagent; 10373283; Life Technologies). For STAT inhibition, fludarabine was added at 50 mM 24 h before cytokine treatment in WT organoid. For STAT3 depletion, Stat3fl/fl Villin-cre organoids were treated with hydroxytamoxifen 24 h before cytokine treatment.
Mouse recombinant il 11
Manufactured by R&D Systems
Mouse recombinant IL-11 is a cytokine that promotes the growth and differentiation of various cell types, including megakaryocytes, hematopoietic stem cells, and osteoblasts.
Automatically generated - may contain errors
Lab products found in correlation
Ifn γ, by Thermo Fisher Scientific (1 mentions)
Anti ifn γ, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Anti cd3 145 2c11, by Thermo Fisher Scientific (1 mentions)
Anti cd28 37.51, by Thermo Fisher Scientific (1 mentions)
Tgf β, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse recombinant il 11
1
Intestinal Organoid Cytokine Treatment
2
Th17 Skewing of Murine Lymph Node Cells
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Lymph nodes (axillary, cervical and inguinal) were harvested from C57BL6/J mice, were pooled, and were dissociated into a single cell suspension. Lymph node cells were resuspended in complete RPMI containing no added cytokines (Th0 conditions) or under Th17-skewing conditions (with added IL-6, TGF-β and anti-IFN-γ, all reagents from eBioscience) in the presence or absence of mouse recombinant IL-11 (R&D systems, Minneapolis, MN). Cells were then plated (0.5×106/well) in 96-well flat-bottom plates that were pre-coated with anti-CD3 (145-2C11) and anti-CD28 (37.51) (both from eBioscience). The proliferation of, and cytokine productions by these cells were measured as described above.
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