Diphenyl sulfoxide

Adenine hemisulfate salt ≥99%, TMAO, DMSO, DCA, UDCA, sulfolane ≥99%, benzophenone, diphenyl sulfoxide, and ThT were purchased from Sigma-Aldrich (Rehovot, Israel). 1,4-Thioxane-1,1-dioxide ≥98% and tetrahydrothiophene 1-oxide were purchased from Tzamal D-Chem (Petah Tikva, Israel). ProteoStat dye was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). DCA, UDCA, 1,4-Thioxane-1,1-dioxide, benzophenone and diphenyl sulfoxide stocks were prepared and diluted in pure DMSO. TMAO and tetrahydrothiophene 1-oxide stocks were prepared and diluted in PBS.

The reagents: 3-amino-1,2,4-triazole, acetylacetone, 1,3-diphenyl-1,3-propanedione, 2,2,6,6-tetramethyl-3,5-heptanedione, 6-methyl-2,4-heptanedione, diphenyl sulfoxide, tetrahydrothiophene-1-oxide, and K₂PtCl₄ were purchased from Sigma Aldrich (St. Louis, MO, United States), whereas the inorganic salts and solvents of analytical grade from Avantor Performance Materials Poland S.A. (Gliwice, Poland). All chemicals and solvents obtained commercially were used without further purification.
The 5,7-disubstituted derivatives of 1,2,4-triazolo[1,5-a]pyrimidine were obtained according to the Bülow and Haas [19 (link),20 (link)] method by the condensation of 3-amino-1,2,4-triazole with corresponding diketones such as: acetylacetone for (dmtp), 6-methyl-2,4-heptanedione for (ibmtp), 2,2,6,6-tetramethyl-3,5-heptanedione for (dbtp) and 3,5-diphenyl-1,3-propanedione for (dptp).