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2 protocols using k3po4

1

Preparation of Metal-Chitosan Composites

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l-Valine (Val), phosphoric acid, NaOH,
FeCl2, FeCl3, ethanol, K2SO4, K3PO4, K2CO3, Hg(NO3)2, AgNO3, ZnCl2, CuSO4, CdCl2, BaCl2, Pb(NO3)2, MnCl2, CaCl2, MgCl2, Cr2(SO4)3, and K2Cr2O7 were bought from Sinopharm Chemical Reagent Co. Ltd.
(Beijing, China).
Poly(ethylene oxide) (average Mw 900 kDa)
and Chitosan (average Mw 200 kDa) were
obtained from Sigma-Aldrich. Dialysis tubing with a flat diameter
of 28 mm and a molecular weight cutoff (MWCO) of 1 kDa were provided
by Biotopped (Viskase). Deionized water with a resistivity of 18.2
MΩ was provided by a GWA-UN water purification system. All materials
and chemicals were of analytical grade and used as received without
further purification.
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2

HPLC Analysis of Chrysanthemum Extracts

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Chrysanthemum morifolium from Anhui Province was purchased from Shanghai Dedatang Sinopharm Co., Ltd. (Shanghai, China). The standard of ICGA (purity >98% by HPLC) was purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). (Bmim)Br (1‐butyl‐3‐methylimidazolium bromide) was purchased from Shanghai Cheng Jie Chemical Co., Ltd. (Shanghai, China). (NH4)2SO4, K3PO4, NaH2PO4, K2CO3, methanol, and ethanol were of analytical grade and were acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Other reagents were all of analytical grade and used without further treatment. Deionized water was obtained with a Milli‐Q water purification system (Millipore) to prepare the sample solutions. The HPLC equipment used was Agilent 1260 HPLC system (Agilent Technologies, Germany).
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