Texas red

Manufactured by PerkinElmer

Texas red is a fluorescent dye commonly used in biological research applications. It has a bright red emission spectrum, making it useful for labeling and detection of various biomolecules.

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Lab products found in correlation

Anti epcam, by Abcam (1 mentions) Opal fluorophores, by PerkinElmer (1 mentions) Anti tmprss2, by Abcam (1 mentions) Anti sars cov 2 nucleoprotein, by Sino Biological (1 mentions) Fluorescein isothiocyanate (fitc), by PerkinElmer (1 mentions) Anti ace2, by Abcam (1 mentions) Fluorescence mounting medium, by Agilent Technologies (1 mentions) Cytovision software, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) High pure plasmid isolation kit, by Roche (1 mentions)

2 protocols using texas red

Multimodal IHC for COVID-19 Markers

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Formalin (4%) fixed duodenum and colon tissue samples were embedded in paraffin, and a 4 μm section of each was obtained on a glass slide. These sections were deparaffinized and incubated with anti-HIV p24 (clone: Kal-1, Dako), anti–SARS-CoV-2 nucleoprotein (clone: 40143-T-62, Sino Biological) anti-ACE2 (clone: ab15348; Abcam), anti-TMPRSS2 (clone: ab109131; Abcam), and anti-EpCAM (clone: ab71916; Abcam) followed by a secondary antibody incubation using the Opal 4-color manual IHC (PerkinElmer) as instructed by the manufacturer. For Opal fluorophores (PerkinElmer), FITC (product number FP1487001KT; PerkinElmer) was used for EpCAM and p24, Texas red (product number FP1488001KT; PerkinElmer) for ACE2, and Cy5 (product number FP1497001KT; PerkinElmer) for TMPRSS2 and SARS-CoV-2 nucleoprotein signal generation. DAPI was used as the nuclear counterstain. The sections were mounted with the Fluorescence Mounting Medium (catalog number S302380-2; Agilent Technologies) and cover-slipped, and the edges were sealed with nail polish. The slides were stored at 2°C–8°C until images were acquired.

Fardoos R., Asowata O.E., Herbert N., Nyquist S.K., Zungu Y., Singh A., Ngoepe A., Mbano I.M., Mthabela N., Ramjit D., Karim F., Kuhn W., Madela F.G., Manzini V.T., Anderson F., Berger B., Pers T.H., Shalek A.K., Leslie A, & Kløverpris H.N. (2021). HIV infection drives interferon signaling within intestinal SARS-CoV-2 target cells. JCI Insight, 6(16), e148920.

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Interphase FISH for Gene Fusions

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Bacterial artificial chromosome (BAC) probes for the UQCR10 (clone RP11-772E21), C1orf194 (clone RP11-695F11), DPYSL2 (clone RP11-1G11), and GFAP (clone RP11-643P22) genes were retrieved from the Human 32K BAC Re-Array library (BacPac Resource Center, Oakland, CA) (Osoegawa, 2001) . Two probes overlapping the CLU locus (clones RP11-810P7 and RP11-16P20; BacPac Resource Center, https://bacpac.chori.org) were used. The clones were selected based on their mapping to the chromosomal sites of the putative gene fusions. Clones were grown in selective media according to the manufacturer's protocol and BAC DNA was extracted using the High Pure Plasmid isolation kit (Roche, Basel, Switzerland). The DNA probes were labelled by nick translation using fluorescein isothiocyanate (FITC) and Texas Red (Perkin Elmer, Waltham, MA) as described previously (Nyquist et al., 2011) . Interphase FISH was performed on fresh slides using frozen tumor cell suspension and the slides were counterstained using 4',6-diamidino-2-phenylindole (DAPI;
Vector Laboratories, Burlingame, USA). CytoVision software (Leica biosystems, Wetzlar, Germany) was used to analyze the FISH images.

Olsen T.K., Panagopoulos I., Gorunova L., Micci F., Andersen K., Kilen Andersen H., Meling T.R., Due-Tønnessen B., Scheie D., Heim S, & Brandal P. (2016). Novel fusion genes and chimeric transcripts in ependymal tumors. Genes, chromosomes & cancer, 55(12).

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