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Raw blue isg cells

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RAW-Blue ISG cells are a reporter cell line derived from murine RAW 264.7 macrophages. These cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the ISG54 (Interferon-Stimulated Gene 54) promoter, which is induced by type I interferons.

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Quanti blue reagent, by InvivoGen (1 mentions)

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RAW-Blue cells (71 citations) RAW-Blue (17 citations) RAW-Blue ISG (2 citations) RAW-ISG cells (2 citations)

2 protocols using raw blue isg cells

1

Quantifying cGAMP Bioactivity

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cGAMP activity was measured using RAW-Blue ISG cells (InvivoGen) that were cultured in DMEM supplemented with 10% FBS, 4.5 g/L glucose, 2mM L-Glutamine, and 100 μg/mL Normacin. Zeocin (200 μg/mL) was added every other passage to maintain selection pressure. RAW-Blue ISG cells were seeded at a density of 50,000 cells/well in a 96 well plate and then treated with the indicated formulations and concentrations for 24 hours. Relative expression of IFN-I was measured using QUANTI-Blue reagent (InvivoGen).
Shae D., Baljon J.J., Wehbe M., Christov P.P., Becker K.W., Kumar A., Suryadevara N., Carson C.S., Palmer C.R., Knight F.C., Joyce S, & Wilson J.T. (2020). Co-Delivery of Peptide Neoantigens and Stimulator of Interferon Genes (STING) Agonists Enhances Response to Cancer Vaccines. ACS nano, 14(8), 9904-9916.
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2

Quantification of ISGF3 Reporter Activity

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The RAW-Blue ISG macrophage cell line stably transfected with an ISGF3-dependent reporter construct was cultured and maintained according to the manufacturer’s instructions (RAW-Blue ISG cells, Invivogen raw-isg). ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
O’Neil J.D., Bolimowska O.O., Clayton S.A., Tang T., Daley K.K., Lara-Reyna S., Warner J., Martin C.S., Mahida R.Y., Hardy R.S., Arthur J.S, & Clark A.R. (2023). Dexamethasone impairs the expression of antimicrobial mediators in lipopolysaccharide-activated primary macrophages by inhibiting both expression and function of interferon β. Frontiers in Immunology, 14, 1190261.
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