Id4 fish probe

Manufactured by Empire Genomics

Sourced in United States

The ID4 FISH Probe is a fluorescent in situ hybridization (FISH) probe designed to detect the presence of the ID4 gene. The probe is used for the identification and localization of the ID4 gene within chromosomal preparations.

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FISH probes (13 citations)

2 protocols using id4 fish probe

Genomic Analysis of ID4 Expression in Breast Cancer

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Tissue sections were analysed using fluorescent in situ hybridisation (FISH) to examine the genomic region encoding ID4 (6p22.3). ID4 FISH Probe (Orange 552–576 nm, Empire Genomics, NY, USA) was compared to the control probe CEP6 (Chromosome 6, Green 5-Fluorescein dUTP). This CEP6 probe marks a control region on the same chromosome as ID4 and is used to normalise ID4 copy number. Breast pathologist Dr. Sandra O’Toole oversaw the FISH quantification for all samples.

Baker L.A., Holliday H., Roden D., Krisp C., Wu S.Z., Junankar S., Serandour A.A., Mohammed H., Nair R., Sankaranarayanan G., Law A.M., McFarland A., Simpson P.T., Lakhani S., Dodson E., Selinger C., Anderson L., Samimi G., Hacker N.F., Lim E., Ormandy C.J., Naylor M.J., Simpson K., Nikolic I., O’Toole S., Kaplan W., Cowley M.J., Carroll J.S., Molloy M, & Swarbrick A. (2020). Proteogenomic analysis of Inhibitor of Differentiation 4 (ID4) in basal-like breast cancer. Breast Cancer Research : BCR, 22, 63.

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Quantifying ID4 Expression in Breast Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue sections were analysed using Fluorescent In Situ Hybridisation (FISH) to examine the genomic region encoding ID4 (6p22.3). ID4 FISH Probe (Orange 552 nm-576 nm, Empire Genomics, NY, USA)
was compared to the control probe CEP6 (Chromosome 6, Green 5-Fluorescein dUTP). This CEP6
probe marks a control region on the same chromosome as ID4 and is used to normalise ID4 copy number. Breast pathologist Dr Sandra O'Toole oversaw the FISH quantification for all samples.

Baker L.A., Holliday H., Roden D., Krisp C., Wu S.Z., Junankar S., Sérandour A.A., Mohammed H., Nair R., Sankaranarayanan G., Law A.M.K., McFarland A., Simpson P.T., Lakhani S.R., Dodson E., Selinger C., Anderson L., Samimi G., Hacker N.F., Lim E., Ormandy C.J., Naylor M.J., Simpson K.J., Nikolić I., O’Toole S.A., Kaplan W., Cowley M.J., Carroll J.S., Molloy M.P., & Swarbrick A. (2020). Proteogenomic analysis of Inhibitor of Differentiation 4 (ID4) in basal-like breast cancer.

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