Ethylene diamine tetraacetic acid (edta)

Dulbecco’s modified Eagle’s medium (DMEM), 10× phosphate-buffered saline, 10× Tris-buffered saline, and Hank’s balanced salt solution (HBSS) were purchased from Welgene, Inc. (Deagu, Korea). Fetal bovine serum (FBS), 2’,7’-dichlorodihydroflorescein diacetate (H₂DCFDA), and 2’,7’-dichlorofluorescein were purchased from Invitrogen, Inc. (Carlsbad, CA, USA). Trypsin-ethylenediaminetetraacetic acid (EDTA) and antibiotics (penicillin and streptomycin) were obtained from Gibco BRL (Grand Island, NY, USA). Sodium phosphate-monobasic, sodium phosphate-dibasic, acetic acid, potassium phosphate-monobasic, and potassium phosphate-dibasic were purchased from Daejung, Ltd. (Chungwon, Korea). 4,6-Dihydroxy-2-mercaptopyrimidine was purchased from Alfa Aesar (Seoul, Korea). EDTA was purchased from Junsei Chemical Co, Ltd. (Tokyo, Japan). L-glutamic acid, dimethyl sulfoxide, sodium dodecyl sulfate (SDS), β-nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium salt hydrate, 4,6-dihydroxy-2-mercaptopyrimidine (DTNB), glutathione reductase (GR, type Ⅲ from baker’s yeast), and L-glutathione reduced (GSH) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).