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Cd117 pecy7

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The CD117/PeCy7 is a fluorescently-labeled monoclonal antibody that binds to the CD117 antigen, also known as c-Kit. It is commonly used in flow cytometry applications to identify and characterize cells expressing the CD117 surface marker.

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Lab products found in correlation

Cd11b percp cy5, by Thermo Fisher Scientific (2 mentions) Cd45.1 pe, by Thermo Fisher Scientific (2 mentions) Moflow, by Beckman Coulter (2 mentions) Canto 2, by BD (2 mentions) Annexin 5 apc, by Thermo Fisher Scientific (2 mentions) Streptavidin pecy5, by BD (2 mentions) Cd4 pe or pb, by Thermo Fisher Scientific (2 mentions) Cd8 pecy7 or apc, by Thermo Fisher Scientific (2 mentions) Cd279 bv421, by Thermo Fisher Scientific (2 mentions) Cd117 apccy7, by Thermo Fisher Scientific (2 mentions) Sca 1 apc, by Thermo Fisher Scientific (2 mentions) Cd45.2 apc, by Thermo Fisher Scientific (2 mentions) Apc brdu flow kit, by BD (2 mentions) Ki67 pe, by Thermo Fisher Scientific (2 mentions) B220 apc cy7, by Thermo Fisher Scientific (2 mentions) Cd19 apc, by Thermo Fisher Scientific (2 mentions) Tcrβ pe, by Thermo Fisher Scientific (2 mentions) Gr 1 pe, by Thermo Fisher Scientific (2 mentions)

2 protocols using cd117 pecy7

1

Multi-lineage Hematopoietic Cell Analysis

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Total white blood cells from hematopoietic organs were stained in PBS supplemented with 2% FBS with fluorochrome-conjugated mouse antibodies against specific hematopoietic lineage markers. For the analysis of transplant-receiving mice, WBM was stained with fluorochrome-conjugated mouse antibodies to discriminate cells derived from competitors and donors (CD45.1+ and CD45.2+ respectively) and GFP expression was used to precisely define donor-derived cells (GFP+ CD45.2+). Fluorochrome-conjugated mouse antibodies were obtained from Becton Dickinson (Streptavidin/PeCy5, CD117/PeCy7, CD45.1/PE, CD4/PE or PB, CD8/PeCy7 or APC, CD19/APC, B220/APCCy7, TCRβ/PE, CD279/BV421, CD11b/PerCP-Cy5.5, Annexin V/APC, Ki67/PE) and eBiosciences (CD117/APCCy7, Sca-1/APC, CD45.2/APC, Gr1/PE). Hoescht 33342 was obtained from Invitrogen. APC BrdU Flow kit (Becton Dickinson) was used according to the manufacturer’s instruction. Cell sorting was performed either on a MoFlow (Beckman Coulter) or Influx (Becton Dickinson) cell sorter and analysis on a Canto II (Becton Dickinson). FACS data were analyzed by FlowJo Software (v8.8.7).
Scourzic L., Couronné L., Pedersen M.T., Della Valle V., Diop M., Mylonas E., Calvo J., Mouly E., Lopez C.K., Martin N., Fontenay M., Bender A., Guibert S., Dubreuil P., Dessen P., Droin N., Pflumio F., Weber M., Gaulard P., Helin K., Mercher T, & Bernard O.A. (2016). DNMT3AR882H mutant and Tet2 inactivation cooperate in the deregulation of DNA methylation control to induce lymphoid malignancies in mice. Leukemia, 30(6), 1388-1398.
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2

Multi-lineage Hematopoietic Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total white blood cells from hematopoietic organs were stained in PBS supplemented with 2% FBS with fluorochrome-conjugated mouse antibodies against specific hematopoietic lineage markers. For the analysis of transplant-receiving mice, WBM was stained with fluorochrome-conjugated mouse antibodies to discriminate cells derived from competitors and donors (CD45.1+ and CD45.2+ respectively) and GFP expression was used to precisely define donor-derived cells (GFP+ CD45.2+). Fluorochrome-conjugated mouse antibodies were obtained from Becton Dickinson (Streptavidin/PeCy5, CD117/PeCy7, CD45.1/PE, CD4/PE or PB, CD8/PeCy7 or APC, CD19/APC, B220/APCCy7, TCRβ/PE, CD279/BV421, CD11b/PerCP-Cy5.5, Annexin V/APC, Ki67/PE) and eBiosciences (CD117/APCCy7, Sca-1/APC, CD45.2/APC, Gr1/PE). Hoescht 33342 was obtained from Invitrogen. APC BrdU Flow kit (Becton Dickinson) was used according to the manufacturer’s instruction. Cell sorting was performed either on a MoFlow (Beckman Coulter) or Influx (Becton Dickinson) cell sorter and analysis on a Canto II (Becton Dickinson). FACS data were analyzed by FlowJo Software (v8.8.7).
Scourzic L., Couronné L., Pedersen M.T., Della Valle V., Diop M., Mylonas E., Calvo J., Mouly E., Lopez C.K., Martin N., Fontenay M., Bender A., Guibert S., Dubreuil P., Dessen P., Droin N., Pflumio F., Weber M., Gaulard P., Helin K., Mercher T, & Bernard O.A. (2016). DNMT3AR882H mutant and Tet2 inactivation cooperate in the deregulation of DNA methylation control to induce lymphoid malignancies in mice. Leukemia, 30(6), 1388-1398.
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