Blood samples from the baseline visit were processed within 4 hours for analysis by flow cytometry. For lymphocyte phenotyping, ethylenediaminetetraacetic acid whole blood was stained for CD3, CD4, CD8, CD45, CD16, CD56, and CD19, as previously described (30 (link)). For B-cell phenotyping, PBMCs were isolated from lithium heparin whole blood by Ficoll gradient density centrifugation. One million PBMCs were incubated with the following antibodies: CD19-VioGreen, anti-IgD-VioBlue, CD24-PerCP-Vio700, CD38-FITC, CD27-APC, CD86-PE-Vio770, CD21-APC-Vio770, and anti-IgM-PE (Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were measured using a FACSLyric flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). Data were analyzed using the FACSSuite (BD Biosciences). The gating strategy is shown in Supplemental Figure 1
Anti igm pe
Manufactured by Miltenyi Biotec
Sourced in Germany
Anti-IgM-PE is a laboratory reagent used for the detection and quantification of IgM antibodies in biological samples. It consists of a phycoerythrin (PE) fluorescent dye conjugated to an anti-IgM antibody, which allows for the specific labeling and identification of IgM-positive cells or molecules.
Automatically generated - may contain errors
Lab products found in correlation
Facssuite, by BD (2 mentions)
Anti igd vioblue, by Miltenyi Biotec (2 mentions)
Cd21 apc vio770, by Miltenyi Biotec (2 mentions)
Cd38 fitc, by Miltenyi Biotec (2 mentions)
Cd19 viogreen, by Miltenyi Biotec (2 mentions)
Facslyric flow cytometer, by BD (2 mentions)
Cd27 apc, by Miltenyi Biotec (2 mentions)
Cd86 pe vio770, by Miltenyi Biotec (2 mentions)
Cd24 percp vio700, by Miltenyi Biotec (2 mentions)
2 protocols using anti igm pe
1
Immunophenotyping of Lymphocytes and B-cells
2
Comprehensive Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood samples from the baseline visit were processed within 4 h for analysis by flow cytometry. For lymphocyte phenotyping, ethylenediaminetetraacetic acid whole blood was stained for CD3, CD4, CD8, CD45, CD16, CD56, and CD19. For immune cell phenotyping, PBMCs were isolated from lithium heparin whole blood by Ficoll gradient density centrifugation. One million PBMCs were incubated with the following antibodies: CD19-VioGreen, anti-IgD-VioBlue, CD24-PerCP-Vio700, CD38-FITC, CD27-APC, CD86-PE-Vio770, CD21-APC-Vio770, and anti-IgM-PE (Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were measured using a FACSLyric flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). Data were analyzed using FACSSuite (BD Biosciences).
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