Taxifolin (CAT# C13293876) and Lipopolysaccharide (LPS) (CAT. No. C15166619) acquired from Shanghai Macklin Biochemical Co, Ltd China (CAT# C13293876). The local pharmaceutical company provided the dimethyl sulfoxide (DMSO) and fluoxetine. It is commonly prescribed to treat various mental health conditions, primarily depression Proteinase K and Phosphate buffer saline (PBS) was supplied by (MP Bio USA). Santa Cruz Biotechnology USA delivered mouse monoclonal anti-TNF Alpha (SC–52B83), mouse monoclonal anti-p-NFKB (SC-271908), 3,3′-diaminoben-Zidine peroxidase (DAB). Hydrogen peroxide (H2O2) (from BDH Germany), Abcam UK supplied the xylene and mountain media. Formaldehyde, Glutathione-S-transferase (GST) (CAT # CS0410), catalase, and reduced glutathione (GSH), tricholoroacetic acid (TCA), as well as other substances CDNB (1-chlor-2,4-di-nitobenzene) (Pub-chem CID:6), was obtained from (Sigma-Aldrich, United States). The Abcam (Cambridge, UK) provided us with a horseradish peroxidase-conjugated secondary antibody (ab-6789). The ELISA kit COX-2 (CAT# PRS-30205Ra) and the ELISA kit PPAR gamma (CAT# E-EL-R0724) were supplied by Elabscience.
Mouse monoclonal anti p nfkb
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Mouse monoclonal anti-p-NFKB is an antibody that specifically recognizes the phosphorylated form of the NFKB protein. It is a research-use only product intended for immunological applications.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffer saline (pbs), by MP Biomedicals (1 mentions)
Ab6789, by Abcam (1 mentions)
Proteinase k, by MP Biomedicals (1 mentions)
Anti 4e bp1, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti nrf2, by Santa Cruz Biotechnology (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
Mouse monoclonal anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg hrp, by Fortis Life Sciences (1 mentions)
Anti p 4ebp1, by Cell Signaling Technology (1 mentions)
Anti eif2α, by Cell Signaling Technology (1 mentions)
2 protocols using mouse monoclonal anti p nfkb
1
Taxifolin and LPS-Induced Inflammatory Modulation
2
Western Blot Antibody Optimization
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To evaluate protein expression on western blot membranes the following antibody were used: rabbit polyclonal anti-p62 (1:1000) (Cell Signaling, 5114T), mouse monoclonal anti-NRF2 (1:100) (Santa Cruz Biotechnology Inc., sc-365,949), mouse monoclonal anti-NQO1 (1:100) (Santa Cruz Biotechnology Inc., sc-32,793), rabbit polyclonal anti p-eIF2α (Cell Signaling, 9721S), rabbit polyclonal anti-eIF2α (Cell Signaling, 9722S), rabbit polyclonal anti-p-4E-BP1 (1:500) (Cell Signaling, 2855T), rabbit polyclonal anti-4E-BP1 (1:500) (Cell Signaling, 9452S), mouse monoclonal anti-p-STAT3 (1:100) (BD Bioscience, 612,356), mouse monoclonal anti-STAT3 (1:500) (BD Bioscience, 610,189), mouse monoclonal anti-p-NF-kB (1:100) (Santa Cruz Biotechnology Inc. sc-101,752), mouse monoclonal anti-NF-kB (1:100) (Santa Cruz Biotechnology Inc., sc-136,548) and mouse monoclonal anti-MBP (1:100) (Santa Cruz Biotechnology Inc., sc-271,524). Mouse monoclonal anti-β-actin (1:5000) (Sigma Aldrich) and mouse monoclonal anti-GAPDH (1:500) (Santa Cruz Biotechnology Inc. sc-47,724) were used as loading control. The goat anti-mouse IgG-HRP (1:10,000) (Bethyl Laboratories, A90-116P) and goat anti-rabbit IgG-HRP (1:10,000) (Bethyl Laboratories, A120-101P) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS-0.1% Tween20 solution containing 3% of BSA (SERVA).
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