The sphere assay was performed as described previously (22 (link)). Briefly, colon cancer cells were plated in ultralow attachment plates (Corning) in X-VIVO medium (Lonza) at a density of 1,000–5,000 viable cells/well. Colon cancer cells were treated with recombinant IL33 (PeproTech) or IL33-conditioned macrophages for different time points. The NFκB inhibitor, BAY11-7082; the P38 inhibitor, SB203580; the JNK inhibitor, SP600125; the MEK/ERK inhibitor, PD98059 (Cayman Chemicals), and the iNOS inhibitor, SMT (Aladdin), and anti-human ST2 antibody (R&D Systems) were added in sphere culture for 1 week. Spheres (>20 μm) were counted.
Anti human st2 antibody
Manufactured by R&D Systems
Sourced in United States
The Anti-human ST2 antibody is a laboratory reagent used in research applications. It binds to the ST2 protein, which is a member of the interleukin-1 receptor family. The antibody can be used for the detection and quantification of ST2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Sp600125, by Cayman Chemical (3 mentions)
Recombinant il 33, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Lonza (2 mentions)
Six well plate, by Corning (2 mentions)
Bay 11 7082, by Cayman Chemical (1 mentions)
Pd98059, by Cayman Chemical (1 mentions)
Sb203580, by Cayman Chemical (1 mentions)
Ultra low attachment plate, by Corning (1 mentions)
X vivo medium, by Lonza (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti human st2 antibody
1
Colon Cancer Spheroid Assay with IL33
2
IL-33 regulates RCC proliferation
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RCC cells were plated in six well plates (Corning, USA) in RPMI1640 medium (Lonza, Switzerland) at a density of 1, 000-5, 000 viable cells/well. RCC cells were treated with recombinant IL-33 (Peprotech, USA) at different doses. The JNK inhibitor, SP600125 (Cayman, USA), or anti-human ST2 antibody (Clone # 97203, R&D systems, USA) were added. The proliferation was measured by CCK8.
3
Modulating RCC Cell Apoptosis via IL-33
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RCC cells were plated in six well plates (Corning) in RPMI1640 medium (Lonza) at a density of 1, 000-5, 000 viable cells/well in the presence of 5-Fu at different doses. RCC cells were treated with 50 ng/ mL recombinant IL-33 (Peprotech). The JNK inhibitor, SP600125 (10 µg/mL, Cayman, USA), or anti-human ST2 antibody (1 µg/mL, R&D systems) were added. The apoptosis rate was measured by flow cytometry.
siRNA Transfection ST2 expression was knockdown using a synthetic siRNA sequence against ST2. RCC cells were transfected with human ST2 siRNAs or control siRNA using Lipofectamine RNAi MAX (Life Technologies) according to manufacturer's protocols.
siRNA Transfection ST2 expression was knockdown using a synthetic siRNA sequence against ST2. RCC cells were transfected with human ST2 siRNAs or control siRNA using Lipofectamine RNAi MAX (Life Technologies) according to manufacturer's protocols.
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