Skov3

Jurkat T lymphoma cells were kindly provided by Dr. Lapointe Réjean (CRCHUM), while MCF-7, SKOV3, and all PC cell lines (22Rv1, LNCaP, DU145, and PC3) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Jurkat T lymphoma cell and PC cells were maintained in RPMI 1640 medium (Wisent Inc., St-Bruno, QC, Canada), MCF-7 was grown in DMEM medium (Wisent Inc.), and SKOV3 in OSE medium (Wisent Inc.). All culture media were supplemented with 10% fetal bovine serum (FBS) (Gibco^®, Thermo Fisher Scientific, Waltham, MA, USA), 0.454 μg/mL of amphotericin B (Wisent Inc.), and 90 μg/mL gentamycin sulfate (Wisent Inc.).

Human ovarian cancer cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA) and cultured in Dulbecco's modified Eagle's medium (SKOV3, CaOV3) or RPMI-1640 (OVCAR8, HeyA8) supplemented with 5 % fetal bovine serum (FBS; Wisent). Early-passage cell lines (designated “iOvCa”) are derived from ascites cultures (designated “EOC”) of the corresponding number (e.g., iOvCa201 is a line derived from EOC201). Early-passage lines were maintained in DMEM/F12 medium (Gibco/Invitrogen, Carlsbad, CA) supplemented with 10 % FBS. The iOvCa147-E2 line is a clone of iOvCa147. To establish stable expression of eGFP-LC3B, sub-confluent (~60-70 %) OVCAR8 cells were transfected with the pBMN-ires-puro-eGFP-LC3B construct (gift of Dr. C. McCormick, Dalhousie University) and transferred to Puromycin-containing selection medium (1 μg/mL) where clones with robust eGFP-LC3B expression were isolated. All cells were maintained in a 37 °C humidified atmosphere of 95 % air and 5 % CO₂. Adherent cells were maintained on tissue culture-treated polystyrene (Sarstedt, Newton, NC) and non-adherent cells were maintained on Ultra-low Attachment (ULA) cultureware (Corning, Corning, NY).