According to the lentivirus transduction instructions, 5 μL negative control (NC) inhibitor lentivirus or miR-130b inhibitor lentivirus (0.1 μM) (Shanghai GenePharma Co. Ltd, Shanghai, China) were mixed with 10 μL co-infective reagent, which was allowed to stand at ambient temperature for 15 min. Anisomycin (HY-18982, MedChemExpress, NJ), a p38/MAPK signaling pathway agonist, was dissolved in dimethylsulfoxide (DMSO) at a concentration of 15 mg/mL. At 24 h before SAH model establishment, 15 μL mixed NC inhibitor solution, 15 μL mixed miR-130b inhibitor solution, and 20 μL Anisomycin solution were injected into the ventricles of SD rats, respectively. Rats were randomized into sham (intraventricular injection of 15 μL normal saline and 20 μL DMSO), SAH (intraventricular injection of 15 μL normal saline and 20 μL DMSO), SAH + NC inhibitor (intraventricular injection of 15 μL mixed NC inhibitor solution and 20 μL DMSO), SAH + miR-130b inhibitor (intraventricular injection of 15 μL mixed miR-130b inhibitor solution and 20 μL DMSO), and SAH + miR-130b inhibitor + Anisomycin group (intraventricular injection of 15 μL mixed miR-130b inhibitor solution and 20 μL Anisomycin solution).44 (link)
Anisomycin hy 18982
Manufactured by MedChemExpress
Sourced in China
Anisomycin (HY-18982) is a natural product isolated from the bacterium Streptomyces griseolus. It is a protein synthesis inhibitor that binds to the 60S ribosomal subunit, preventing the formation of peptide bonds and thereby inhibiting translation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anisomycin hy 18982
1
Lentivirus-mediated miR-130b inhibition and anisomycin treatment in SAH model
2
Pulmonary Artery Smooth Muscle Cell Culture
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Pulmonary artery smooth muscle cells (PASMC) were purchased from the Cell Bank of the Chinese Academy of Sciences, Shanghai, China. DMEM high glucose medium (11995, Gibco, USA) containing 10% fetal bovine serum (YSN0121, ExCell Bio, China) and 1% penicillin-streptomycin mixture (P1400, Solarbio, China) was configured, and the cells were cultured in the configured DMEM medium and placed in an incubator at 37°C with 5% CO2 (CI-191C, Jiemei Electronics, China). The experimental agent 4-HS was provided by Professor S. Zhang (State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China). MCT (HY-N0750), Sesamin (HY-N0121) and Anisomycin (HY-18982) were purchased from Med Chem Express Shanghai, China.
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