The area percent of newly formed bone was estimated using Leica Qwin 500 Image Analyzer Computer System, (Leica Microsystems, Switzerland). The cursor was used to outline the areas of newly formed bone trabeculae, which were then masked by a binary colour that could be measured by the computer. The image analyser is calibrated automatically to convert the measurement units (pixels) produced by the image analyser program into actual micrometre units. The area percent of newly-formed bone was estimated in 8 different fields in each group using magnification (x 100). Mean values and standard deviation (SD) were calculated for each group.
Qwin 500 image analyzer computer system
Manufactured by Leica
Sourced in Switzerland, United Kingdom
The Leica Qwin 500 Image Analyzer Computer System is a specialized laboratory equipment designed for image analysis and processing. It provides comprehensive tools for capturing, analyzing, and quantifying digital images. The system's core function is to enable researchers and analysts to perform detailed measurements, feature extraction, and statistical analysis on a wide range of sample types.
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Lab products found in correlation
4 protocols using qwin 500 image analyzer computer system
1
Quantifying Newly Formed Bone Density
2
Histological Analysis of Induced Furcation Defects
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Nine non-serial histological sections of each block, corresponding to the first three, central and last three sections, which contained notches in the roots were examined with a light microscope under conventional and polarised light to evaluate the areas of new bone formation in the induced furcation defects. Histological analysis was performed using the Leica Qwin 500 Image Analyzer Computer System (Leica Microsystems, Swizerland) with optical magnification of 100 and 200 x on the H & E slides.
The presence of the following reparative criteria was subjectively assessed: new woven bone formation, osteocytes and variability in their size, in addition to attempts of new mature bone (osteon) formation.
The presence of the following reparative criteria was subjectively assessed: new woven bone formation, osteocytes and variability in their size, in addition to attempts of new mature bone (osteon) formation.
3
Collagen Fiber Quantitative Assessment
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A quantitative study was performed using Leica Qwin 500 image analyzer computer system (England) to assess the mean area percent of collagen fibers deposition in Masson's trichrome stained sections as well as the area percent of immunohistochemically positive structures. The image analyzer consisted of a colored video camera, colored monitor, hard disk of IBM personal computer connected to the microscope and controlled by Leica Qwin 500 software.
The image analyzer was first calibrated automatically to convert the measurement units (pixels) produced by image analyzer program into actual measurement units. Digital images were uniformly acquired and snapped at x400 magnification by Olympus IX51 equipped with a DP72 device camera. The obtained data were calculated in six nonoverlapping fields per slide in each group.
The image analyzer was first calibrated automatically to convert the measurement units (pixels) produced by image analyzer program into actual measurement units. Digital images were uniformly acquired and snapped at x400 magnification by Olympus IX51 equipped with a DP72 device camera. The obtained data were calculated in six nonoverlapping fields per slide in each group.
4
Evaluating Tooth Root Resorption and Cementum
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Tissue blocks were dissected and stored in 4% paraformaldehyde. After the samples were air dried for 30 minutes, microcomputed tomography scanning was performed using SkyScan® 1076 MicroCT scanner and associated software (Version 2.6.0). Reconstructed images were created, and resorption lacunae volumes were measured using CTAn software (SkyScan®) according to the method described before [Figure 1 ].[24 (link)] The total resorption lacunae volume was calculated for each tooth root in each treatment group and then compared. Tissue blocks were prepared for histomorphometric analysis by demineralizing in 10% formic acid and decalcifying in ethylenediaminetetraacetic acid (EDTA), then cutting serial sections (7 μm) in the buccolingual plane through mesiodistal extension and staining with hematoxylin and eosin. Slides were analyzed by light microscopy (Leica Qwin 500 image analyzer computer system [England]) at 40× magnification and photos were then produced. MetaMorph Software (Molecular Devices LLC, California) was used to measure cementum thickness on compression and tension sides at 3 root levels, namely, coronal (level 1), middle (level 2), and apical (level 3) [Figure 2 ]. Sample photomicrographs and micro-CT images from a tooth root from each treatment group and the control group were produced [Figure 3 ]. The assessor was blinded during all data collection.
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