Thymol (purity 98.5%) was obtained from Sigma-Aldrich. Sulfadiazine Polymyxin Sulfite agar (SPS agar), Tween 60 (T60), Tween 80 (T80), triphenyl tetrazolium chloride, violet red bile agar (VRBA), nutrient broth, plate count agar (PCA), sulfadiazine polymyxin sulfite agar (SPS agar), De Man, Rogosa and Sharpe agar (MRS), and yeast extract glucose chloramphenicol agar (YGC) were supplied by Merck Chemical Co., Limited (Darmstadt, Germany). All of the reagents used were of analytical grade. Deionized water was used for the experiments.
Yeast extract glucose chloramphenicol agar
Manufactured by Merck Group
Sourced in Germany
Yeast extract glucose chloramphenicol agar is a culture medium used for the isolation and enumeration of bacteria. It contains yeast extract, glucose, and chloramphenicol as growth-promoting and selective components.
Automatically generated - may contain errors
Lab products found in correlation
M17 agar, by Merck Group (2 mentions)
Thymol, by Merck Group (1 mentions)
Nutrient broth, by Merck Group (1 mentions)
Triphenyl tetrazolium chloride (ttc), by Merck Group (1 mentions)
Plate count agar pca, by Merck Group (1 mentions)
Violet red bile agar, by Merck Group (1 mentions)
Tween 60, by Merck Group (1 mentions)
S aureus, by American Type Culture Collection (1 mentions)
Chitosan, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Mrs agar, by Merck Group (1 mentions)
Ygc agar, by Merck Group (1 mentions)
Anaerobic jar, by Merck Group (1 mentions)
Vancomycin hydrochloride, by Merck Group (1 mentions)
De man rogosa sharpe agar, by Merck Group (1 mentions)
Ringer solution, by Merck Group (1 mentions)
5 protocols using yeast extract glucose chloramphenicol agar
1
Thymol-based Antimicrobial Assay Protocol
2
Antimicrobial Chitosan-Montmorillonite Nanocomposite
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Chitosan was obtained from Sigma-Aldrich (Cat. No. 448869) with 75–85% degree of acetylation and low molecular weight, also, the Montmorillonite nanoclay and acetic acid were obtained from Sigma-Aldrich (Cat. No. 69866) and Merck (Cat. No. 100063) respectively.
Salmonella-Shigella (SS) Agar medium for Salmonella spp., Mannitol salt agar for S. aureus, Violet Red Bile Lactose Agar for coliforms, Lauryl Sulfate Broth and MacConkey agar for the examination of E. coli and Yeast Extract Glucose Chloramphenicol Agar for mold and yeast obtained from Merck (12 ).
The standard microorganisms were supplied by the Ibresco Co, (Iran) comprised E. coli (strain ATCC 19118), S. aureus (ATCC 6538), S. cerevisiae (PTCC 5074), A. brasiliensis and S. enterica (PTCC 1709).
Salmonella-Shigella (SS) Agar medium for Salmonella spp., Mannitol salt agar for S. aureus, Violet Red Bile Lactose Agar for coliforms, Lauryl Sulfate Broth and MacConkey agar for the examination of E. coli and Yeast Extract Glucose Chloramphenicol Agar for mold and yeast obtained from Merck (12 ).
The standard microorganisms were supplied by the Ibresco Co, (Iran) comprised E. coli (strain ATCC 19118), S. aureus (ATCC 6538), S. cerevisiae (PTCC 5074), A. brasiliensis and S. enterica (PTCC 1709).
3
Microbiological Analysis of Kefir
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Microbiological analysis was performed by the traditional plate method [15], according to the following standards: ISO 15214:1998 [16] and ISO 6611:2004 [17] . The number of each type of bacteria was determined with three repetitions. The following microbiological media were used for the analyses: MRS (de Man, Rogosa, and Sharpe) agar (Merck, Darmstadt, Germany), for determining the number of lactobacilli cells; M17 agar (Merck, Darmstadt, Germany), for determining the number of lactococci cells; and YGC (Yeast Extract Glucose Chloramphenicol) agar (Merck, Darmstadt, Germany), for determining the number of yeasts. All media were incubated at 30 • C for 72 h. The Petri dishes with the MRS agar were inserted in the anaerobic jars (Merck, Darmstadt, Germany), those containing the M17 agar were grown aerobically, and Petri dishes with the YGC agar were incubated aerobically for up to 5 days at 25 • C. The results are expressed as mean values of colony-forming unit per mL of kefir sample (CFU/mL) in two parallel replicates, and then as decadic logarithm.
4
Yeast and Mold Enumeration at 17°C
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Preparation was similar as for mesophilic counts but plates were incubated at 17 ºC for 5 days. (ISO 21527:2008) . A 1 mL aliquot of the solution used in the aerobic colony count determination was poured into each of 3 Petri plates, and 15-10 mL of Yeast extract glucose chloramphenicol agar (Merck 1.16000) were then added. When solidified, plates were incubated at 25 °C for 5 days. Colonies were recorded. Enterobacteriaceae (ISO 21528-2:2004) .
5
Enumeration of Kefir Microbiota
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Kefir samples (10 mL) were dispersed with 90 mL of sterile sodium thiosulfate solution (0.2% wt/vol) and homogenized for 1 min using a stomacher (Smasher, AES Chemunex, Bruz, France). Further dilutions were made using Ringer solution (Merck, Darmstadt, Germany). Lactobacilli counts were determined on de Man Rogosa Sharpe agar (Merck) after incubation at 30°C under anaerobic conditions for 5 d (García Fontán et al., 2006) . Lactococci counts were enumerated on M17 Agar (Merck) at 30°C for 3 d (Irigoyen et al., 2005) . Leuconostoc spp. counts were determined on de Man Rogosa Sharpe agar (Merck) incorporated with vancomycin hydrochloride (Sigma-Aldrich, St Louis, MO) at 30°C for 3 d (García Fontán et al., 2006) and yeasts were grown on yeast extract glucose chloramphenicol agar (Merck) at 25°C for 3 d (Magra et al., 2012) .
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