Gas chromatograph model gc 2010

Seeds were collected at seven seed developmental stages: 7, 14, 21, 28, 35, 42, and 49 DAP. Fifty seeds were harvested from the siliques on the main inflorescences at each stage to measure SZ. Based on the Biologische Bundesanstalt, Bundessortenamt, and CHemical (BBCH) industry scale [61 ], six representative plants from the middle of each plot were harvested at growth stage 99 (harvested product) for measurement of SZ, TSW, and SL. The mature seed OC and seed coat transparency were assayed using NIRS (DS2500) with previously established models for Brassica crops [62 ]. The FA composition, specifically the compositions of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid, was analyzed by gas–liquid chromatography on a Perkin Elmer Gas Chromatograph Model GC-2010 (Shimadzu, Kyoto, Japan) equipped with a fused silica capillary column DB-WAX (30 m × 0.25 mm id, 0.25 μm film thickness, J&W, Folsom, CA, USA), as reported previously [63 (link)]. The OC in seeds harvested from seven developmental stages was assayed by GC–MS, as previously described [64 ]. For each trait, at least five independent biological replicates were measured.