Log In Sign up
The largest database of trusted experimental protocols

Lsrii fortessa 2 4 and 5 laser cytometer

Manufactured by FlowJo
Visit Supplier

The LSRII Fortessa II (4 and 5 laser) cytometer is a flow cytometry instrument that can be configured with either 4 or 5 lasers. It is capable of detecting and analyzing multiple parameters of individual cells or particles in a sample.

Automatically generated - may contain errors

Lab products found in correlation

Red blood cell lysis buffer, by BioLegend (2 mentions) α cd16 32 ab clone 93, by BioLegend (2 mentions) Percp cy5.5 clone 30 f11, by BD (2 mentions) Apc cy7 clone 145 2c11, by BioLegend (2 mentions) Alexa700 clone rm4 4, by BioLegend (2 mentions) Pe clone 29f 1a12, by BioLegend (2 mentions)

Spelling variants of this product

Fortessa (11 citations) Fortessa cytometer (3 citations)

2 protocols using lsrii fortessa 2 4 and 5 laser cytometer

1

Tumor-infiltrating Lymphocyte Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pleural tumor nodules were harvested into cold PBS and mechanically disaggregated through 70-μm filters and suspended in PBS. After centrifugation (Thermo-Scientific [Waltham, Mass]; 4°C, 2100 rpm), the supernatant was discarded, and residual tumor was resuspended in red blood cell lysis buffer (1×, BioLegend [San Diego, Calif]); 1.0 × 106 cells per mL were blocked with α-CD16/32 Ab (clone 93; BioLegend: 101319; 1:1000) and stained with antibodies against mouse CD45 (PerCP-Cy5.5; clone 30-F11; BD: 550994;1:200), CD3 (APC-Cy7; clone 145–2C11; BioLegend: 100330; 1:200), CD4 (Alexa700; clone RM4–4; BioLegend: 11,602; 1:200), CD8 (BV785; clone 53–6.7; BioLogend: 1:200), and PD-1 (PE; clone 29F.1A12; BioLegend: 135205; 1:200). Similar procedures were followed to profile circulating lymphocytes in the spleen. Data were collected on a Becton Dickinson (Franklin Lakes, NJ) LSRII Fortessa II (4 and 5 laser) cytometer and analyzed using FlowJo software (v10.6.2). Conventional T-cell and PD-1 gating strategies were completed for all samples and displayed as the percentage positive of all live cells (Figure E1).
Ekeke C.N., Russell K.L., Murthy P., Guo Z.S., Soloff A.C., Weber D., Pan W., Lotze M.T, & Dhupar R. (2020). Intrapleural interleukin-2–expressing oncolytic virotherapy enhances acute antitumor effects and T-cell receptor diversity in malignant pleural disease. The Journal of thoracic and cardiovascular surgery, 163(4), e313-e328.
+ Open protocol
+ Expand
2

Tumor-infiltrating Lymphocyte Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pleural tumor nodules were harvested into cold PBS and mechanically disaggregated through 70-μm filters and suspended in PBS. After centrifugation (Thermo-Scientific [Waltham, Mass]; 4°C, 2100 rpm), the supernatant was discarded, and residual tumor was resuspended in red blood cell lysis buffer (1×, BioLegend [San Diego, Calif]); 1.0 × 106 cells per mL were blocked with α-CD16/32 Ab (clone 93; BioLegend: 101319; 1:1000) and stained with antibodies against mouse CD45 (PerCP-Cy5.5; clone 30-F11; BD: 550994;1:200), CD3 (APC-Cy7; clone 145–2C11; BioLegend: 100330; 1:200), CD4 (Alexa700; clone RM4–4; BioLegend: 11,602; 1:200), CD8 (BV785; clone 53–6.7; BioLogend: 1:200), and PD-1 (PE; clone 29F.1A12; BioLegend: 135205; 1:200). Similar procedures were followed to profile circulating lymphocytes in the spleen. Data were collected on a Becton Dickinson (Franklin Lakes, NJ) LSRII Fortessa II (4 and 5 laser) cytometer and analyzed using FlowJo software (v10.6.2). Conventional T-cell and PD-1 gating strategies were completed for all samples and displayed as the percentage positive of all live cells (Figure E1).
Ekeke C.N., Russell K.L., Murthy P., Guo Z.S., Soloff A.C., Weber D., Pan W., Lotze M.T, & Dhupar R. (2020). Intrapleural interleukin-2–expressing oncolytic virotherapy enhances acute antitumor effects and T-cell receptor diversity in malignant pleural disease. The Journal of thoracic and cardiovascular surgery, 163(4), e313-e328.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!