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Gc grade solvents

Manufactured by Merck Group
Sourced in Germany

GC-grade solvents are high-purity organic solvents designed for use in gas chromatography (GC) applications. These solvents are formulated to meet the stringent requirements of GC analysis, ensuring minimal interference and optimal separation of analytes. They are available in a variety of common GC solvents, such as hexane, acetone, and methanol. GC-grade solvents are manufactured to provide consistent quality and reliability for GC-based analytical techniques.

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2 protocols using gc grade solvents

1

Cytotoxicity Assay of Natural Compounds

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The microbial media were purchased from HiMedia company, India including potato dextrose agar (PDA) and NSA (nutrient-sucrose agar) media. Standard chemicals and GC-grade solvents were purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). All chemicals used for the cytotoxicity assay were purchased from Bio Basic Inc. (Markham, ON, Canada), including RPMI-1640 medium, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), fetal bovine serum, DMSO (Dimethyl sulfoxide), and PBS (Phosphate-Buffered Saline). RNase and propidium iodide were from Abcam (Boston, MA, USA), FITC Annexin V with PI from BioVision, (Milpitas, CA, USA) and RT-PCR kits and oligonucleotide primers from Bio-Rad (Hercules, CA, USA).
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2

Angiotensin I and Fluorescein-BSA Protocol

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Angiotensin I was purchased from Sigma-Aldrich (St. Louis, MO), and fluorescein-labeled BSA was purchased from Rockland Immunochemicals (Limerick, PA). TRI Reagent for use in a single-step isolation of RNA and DNA from cells and tissues and GC-grade solvents used in this study were purchased from Sigma-Aldrich. Laboratory-purified water, the equivalent of Milli-Q water, was used to prepare solvents for HPLC analysis and extractables studies.
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