To quantify eosinophils in BAL fluid, cells were stained with FITC-conjugated anti-CD11b antibodies (eBioscience), APC/Cy7-conjugated anti-CD11c antibodies (eBioscience), and PE-conjugated anti-Siglec-F antibodies (BD Biosciences). To measure IL-23 receptor (IL-23R) expression in lung cells, single cells prepared from lung tissue were stained with PerCP-Cy5.5-conjugated anti-CD45 antibodies, FITC-conjugated anti-F4/80 antibodies (eBioscience), APC/Cy7-conjugated anti-CD11c antibodies, APC-conjugated anti-epithelial cell adhesion molecule (EpCAM) antibodies (eBioscience), and PE-conjugated anti-IL-23R antibodies (eBioscience).
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