Cerebral cortex was dissected from underlying deep white matter, corpus callosum, caudate, and brain stem on DPI 1 into 3mm sections and placed into PAB (PBS, 1% BSA, and 0.1% sodium azide) on ice. Samples were blocked with Trustain FcX (anti-mouse CD16/32; Biolegend 10 μg/mL) and incubated with antibodies for 2 hr at 4°C: PE-conjugated anti-CD31 (#102408 Biolegend 8 μL), APC-conjugated anti-CD45 (#103112 clone 30-F11 Biolegend 5 μL) or APC-conjugated anti-Ly6G (#127613 Biolegend 5 μL). Samples of stained cortex were then washed in PAB, placed on a microscope slide and imaged using a wide-field fluorescence microscope and monochrome CCD digital camera and analyzed using Zen Blue software (Zeiss, Oberkochen, Germany).
Apc conjugated anti ly6g
Manufactured by BioLegend
Sourced in United States
APC-conjugated anti-Ly6G is a flow cytometry antibody that binds to the Ly6G antigen, which is expressed on the surface of neutrophils. It can be used for the identification and enumeration of neutrophils in cell samples.
Automatically generated - may contain errors
Lab products found in correlation
Zen blue software, by Zeiss (1 mentions)
Pe conjugated anti cd31, by BioLegend (1 mentions)
Apc conjugated anti cd45, by BioLegend (1 mentions)
Cytexpert 2, by Beckman Coulter (1 mentions)
Anti cd16 cd32, by BD (1 mentions)
Cytoflex flow cytometer, by Beckman Coulter (1 mentions)
Fitc conjugated anti cd11b, by BD (1 mentions)
2 protocols using apc conjugated anti ly6g
1
Cerebral Cortex Dissection and Immunostaining
2
Quantifying Mac-1 Expression in Neutrophils
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For analysis of Mac-1 expression, freshly isolated bone marrow neutrophils were fixed with 4% formaldehyde and then washed twice with PBS containing 2% FBS. To reduce nonspecific binding, FcγRIII/FcγRII was blocked with anti-CD16/CD32 (553124, BD Bioscience, San Diego, CA, USA), and continued labeling with FITC-conjugated anti-CD11b (553310, BD Biosciences, San Diego, CA, USA) and APC-conjugated anti-Ly6G (127614, Biolegend, London, UK). Flow cytometric detection was performed using the CytoFLEX Flow Cytometer (Beckman Coulter, Indianapolis, IN, USA) and data was analyzed by CytExpert 2.0 software (Beckman Coulter, Indianapolis, IN, USA).
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