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Lomitapide

Manufactured by Selleck Chemicals
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Sourced in United States

Lomitapide is a synthetic small molecule developed for use as a laboratory research tool. It functions as an inhibitor of microsomal triglyceride transfer protein (MTP), a key regulator of lipoprotein assembly. Lomitapide can be used to study the role of MTP in various biological processes.

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Lab products found in correlation

Anti vps34, by Proteintech (2 mentions) Anti phospho beclin 1 ser93, by Cell Signaling Technology (2 mentions) Glutathione s transferase tag protein puri cation kit, by Beyotime (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti beclin 1, by Cell Signaling Technology (2 mentions) Anti ampkα, by Cell Signaling Technology (2 mentions) Anti phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Prescission protease, by Beyotime (2 mentions) Anti atg14, by Proteintech (2 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions) Anti lc3b, by Cell Signaling Technology (2 mentions) Anti cleaved parp, by Cell Signaling Technology (2 mentions) Anti caspase 3, by Cell Signaling Technology (2 mentions) Anti gapdh, by Proteintech (2 mentions) Reactive oxygen species assay kit, by Beyotime (2 mentions) Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (2 mentions) Wst 1 cell proliferation and cytotoxicity assay kit, by Beyotime (2 mentions)

2 protocols using lomitapide

1

Lomitapide Apoptosis and Autophagy Assay

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Drugs, antibodies and reagents Lomitapide was acquired from Selleck Chemicals (Huston, TX, USA) and freshly prepared into 5 mM stock solution with DMSO. Anti-Caspase-3, anti-cleaved caspase-3, anti-cleaved PARP, anti-LC3B, anti-AMPKα, anti-phospho-AMPKα Thr172, anti-phospho-Beclin-1 Ser93, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Vps34, anti-Atg14 and anti-Gapdh antibodies were obtained from Proteintech (Chicago, IL, USA). The WST-1 cell proliferation and cytotoxicity assay kit, glutathione S-transferase (GST) tag protein puri cation kit, reactive oxygen species assay kit and PreScission protease were obtained from Beyotime Biotechnology (Shanghai, China). An Annexin V-FITC/PI apoptosis detection kit was obtained from KeyGen Biotechnology (Nanjing, Jiangsu, China). Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Zuo Q., Liao L., Yao Z.T., Liu Y.P., Wang D.K., Li S.J., Yin X.F., He Q.Y, & Xu W.W. (2021). Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy. Cancer letters, 521.
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2

Lomitapide Apoptosis and Autophagy Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drugs, antibodies and reagents Lomitapide was acquired from Selleck Chemicals (Huston, TX, USA) and freshly prepared into 5 mM stock solution with DMSO. Anti-Caspase-3, anti-cleaved caspase-3, anti-cleaved PARP, anti-LC3B, anti-AMPKα, anti-phospho-AMPKα Thr172, anti-phospho-Beclin-1 Ser93, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Vps34, anti-Atg14 and anti-Gapdh antibodies were obtained from Proteintech (Chicago, IL, USA). The WST-1 cell proliferation and cytotoxicity assay kit, glutathione S-transferase (GST) tag protein puri cation kit, reactive oxygen species assay kit and PreScission protease were obtained from Beyotime Biotechnology (Shanghai, China). An Annexin V-FITC/PI apoptosis detection kit was obtained from KeyGen Biotechnology (Nanjing, Jiangsu, China). Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Zuo Q., Liao L., Yao Z., Liu Y., Wang D., Li S., Yin X., He Q., & Xu W. (2021). Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy.
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