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2 protocols using 50 bp dna ladder

1

DNA Nanostructure Synthesis Protocol

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Dulbecco's Modified Eagle Medium (DMEM) was purchased from Lonza. DMEM-F12, HAMS-F12, Fetal bovine serum (FBS), PenStrap, Trypsin-EDTA (0.25%), collagen were purchased from Gibco and Phosphate buffer saline (PBS) was purchased from HyClone. The sequences used for DNA nanostructure synthesis (Table 12345), 6X loading dye, 50 bp DNA ladder, mowiol, transferrin-A488, Cy5, Hoechst, Pitstop-2, Dynasore and Lactose were ordered from Sigma Aldrich. Nuclease free water, ammonium persulfate, ethidium bromide, TEMED, paraformaldehyde and adherent cell culture dishes were purchased from Himedia. Tris-Acetate EDTA (TAE), Acrylamide/bisacrylamide sol 30% were purchased from GeNei. Magnesium chloride was ordered from SRL, India. Galectin-3 was provided as a gift from Johannes team at Institut Curie, Paris.
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2

Endothelial Cell Culture and DNA Nanostructures

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1. Materials: Human Umbilical Vein Endothelial Cells (HUVECs), Endothelial Cell Growth Basal Medium-2 (EBM) and EGM-2 SingleQuots TM Supplements were obtained from Lonza. Fetal bovine serum (FBS), PenStrap, Trypsin-EDTA (0.25%), collagen was acquired from Gibco. Phosphate buffer saline (PBS) was purchased from HyClone. The sequences used for DNA nanostructure synthesis (Table 1-4), 6X loading dye, 50 bp DNA ladder, mowiol, transferrin-A488, Hoechst and triton-X 100 were ordered from Sigma Aldrich. Nuclease free water, ammonium persulfate, ethidium bromide, TEMED, paraformaldehyde (37%) and adherent cell culture dishes were procured from Himedia. Tris-Acetate EDTA (TAE), Acrylamide/bisacrylamide sol 30% were purchased from GeNei. Magnesium chloride was ordered from SRL, India. Geltrex TM and CellMask TM were obtained from ThermoFischer. Matrigel and collagen were purchased from Corning.
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