Igor pro software package

Following precipitation, the dried protein pellets were resolubilized in 100 μL of 15% acetonitrile and 0.1% formic acid in water (15 min, ultrasonication bath). The entire sample was immediately injected onto a self-packed 30 cm × 0.5 mm POROS R2 column coupled to an Agilent 1200 HPLC system. Solvent A was 0.1% formic acid in water, and solvent B was 0.1% formic acid in acetonitrile. The following gradient was employed: 0 min, 15% B; 5 min, 15% B, 5.01 min, 50% B; 15 The data obtained for intact protein from Orbitrap Velos Pro was deconvoluted using Xcalibur software which accompanies the Orbitrap platform, whereas other data for intact protein were deconvoluted using Microsoft Excel. MS spectra of intact proteins and peptides were plotted using Igor Pro Software package (WaveMetrics, Lake Oswego, OR).

The polymer film surfaces were imaged using an MFP-3D atomic force microscope (AFM) (Asylum Research, CA) with a Mikromash NSC15 cantilever (spring constant ≈ 37
Nm -1 ). Images of the polymer surfaces were obtained using AC mode (5 μm × 5 μm) at a 0.5 Hz scan rate in air. The Asylum Research Analysis software with the Igor Pro Software Package (WaveMetrics, OR) was used to determine the RMS roughness and surface area values.

The polymer film surfaces were imaged using an MFP-3D atomic force microscope (AFM) (Asylum Research, CA) with a Mikromash NSC15 cantilever (spring constant ≈ 37
Nm -1 ). Images of the polymer surfaces were obtained using AC mode (5 μm × 5 μm) at a 0.5 Hz scan rate in air. The Asylum Research Analysis software with the Igor Pro Software Package (WaveMetrics, OR) was used to determine the RMS roughness and surface area values.