Vhx 1000 microscope

Manufactured by Keyence

Sourced in Japan

The VHX-1000 is a digital microscope that captures high-resolution images and video. It features a wide magnification range, advanced optics, and user-friendly software for image analysis and measurement.

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Lab products found in correlation

Vh z20r camera, by Keyence (2 mentions) Vh k20 adapter, by Keyence (2 mentions) Dm2000 microscope, by Leica (1 mentions) Tryptone, by Teknova (1 mentions)

Close spelling variants of this product

VHX-1000 digital light microscope VHX-1000 digital microscope VHX-1000

7 protocols using vhx 1000 microscope

Measuring Plant Morphology and Cell Identity

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Plant areas and perimeters were measured using fiji from images taken using a VHX‐1000 microscope (Keyence, Osaka, Japan) with a ×10 objective, excluding gametophores. These values were used to calculate the perimeter ratio, the ratio between the measured perimeter and the perimeter of a perfectly circular plant of the same area. For cell identity measurements, filaments protruding from the margins of 4‐wk‐old plants were dissected and stained with 0.3% Toluidine Blue for 2 min, rinsed in water and mounted on slides with coverslips before imaging with a DM2000 microscope (Leica, Wetzlar, Germany) using a ×40 objective or a VHX‐1000 microscope (Keyence) using a ×50–200 objective. The length and cell division angle of subapical cells of main filaments and of the second cell in branches with at least three cells were measured using fiji as described previously (Coudert et al., 2019 (link)).

Nemec‐Venza Z., Madden C., Stewart A., Liu W., Novák O., Pěnčík A., Cuming A.C., Kamisugi Y, & Harrison C.J. (2022). CLAVATA modulates auxin homeostasis and transport to regulate stem cell identity and plant shape in a moss. The New Phytologist, 234(1), 149-163.

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Microscopic Characterization of Plant Cells

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Plant areas and perimeters were measured using FIJI from images taken using a Keyence VHX-1000 microscope with a 10 X objective. These values were used to calculate the Perimeter Ratio, the ratio between the measured perimeter and the perimeter of a perfectly circular plant of the same area. For cell identity measurements, filaments protruding from the margins of four week-old plants were dissected and stained with 0.3 % Toluidine Blue for 2 minutes, rinsed in water and mounted on slides with coverslips prior to imaging with a Leica DM2000 microscope using a 40 X objective or a Keyence VHX-1000 microscope using a 50-200 X objective. The length and cell division angle of sub-apical cells of main filaments and of the second cell in branches with at least three cells were measured using FIJI as previously described [28] .

Venza Z.N., Madden C., Stewart A.S., Liu W., Novák O., Pěnčík A., Cuming A.C., Kamisugi Y., & Harrison C.J.O. (2021). PpRPK2 modulates auxin homeostasis and transport to specify stem cell identity and plant shape in the moss Physcomitrella.

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Insect Wing Imaging Methodology

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The procedures applied to capture WIPs were the same as those described for Glossina sp. WIPs acquisition^{21 (link)}. Wings were dissected and deposited on a glass slide, adding a small cover slide. The picture was taken with a Keyence™ VHX 1000 microscope, using the VH-Z20r camera and a Keyence VH K20 adapter, allowing an illumination incidence of 10°. The High Dynamic Range (HDR) function was used for all photos. All pictures were enlarged to a maximal occupancy, making the size of the wing not a discriminating parameter for insect species identification. The numerical parameters settled are the same as described and detailed in the publication on Glossina spp.^{21 (link)}.

Cannet A., Simon-Chane C., Histace A., Akhoundi M., Romain O., Souchaud M., Jacob P., Sereno D., Gouagna L.C., Bousses P., Mathieu-Daude F, & Sereno D. (2023). Wing Interferential Patterns (WIPs) and machine learning for the classification of some Aedes species of medical interest. Scientific Reports, 13, 17628.

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Imaging Techniques for P. litorale

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The specimen of P. litorale is an adult female with a body length of 11 mm measured from the tip of the proboscis to the end of the anal tubercle. It was raised in our laboratory culture of this species (for details, see Ungerer and Scholtz 2009 (link)). The living animal was anaesthetized with CO₂ and photographed from dorsal and ventral perspectives with a Keyence VHX-1000 microscope by combining stacks of images at various z-levels with the implemented software. Afterwards, the animal was fixed in Bouin’s solution (saturated aqueous picric acid, pure acetic acid, and 10 % formaldehyde solution) for several hours at room temperature and subsequently washed and stored in 70 % ethanol. Additional pictures of the fixed specimen were taken with a Zeiss Lumar V12 stereomicroscope equipped with epifluorescence, making use of the green autofluorescence of the animal’s cuticle when excited with blue light.

Scholtz G, & Brenneis G. (2016). The pattern of a specimen of Pycnogonum litorale (Arthropoda, Pycnogonida) with a supernumerary leg can be explained with the “boundary model” of appendage formation. Die Naturwissenschaften, 103, 13.

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Standardized Insect Wing Imaging Protocol

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Insect wings were dissected and deposited on a glass slide. For samples preserved in 70° ethanol, wings were layered overnight at room temperature on a glass slide before being photographed. For image acquisition, a cover slide is deposited on the sample. The picture was taken using a Keyence™ VHX 1000 microscope, the VH-Z20r camera, and a Keyence VH K20 adapter allowing an illumination incidence of 10°. Image acquisition was performed using the High Dynamic Range (HDR) function. To exclude the size as a discriminating parameter of Glossina identification, magnification was adjusted to ensure constant-size pictures.
The numerical parameter settled were as follow:

Camera White Balance: 3200 K

Shutter Speed: preset 1/15(sec)

Gain: 0 dB

Frame rate 15F/s

HDR function:

Brightness: 15%

Texture: 15%

Contrast: 45%

Color: 100%

Next, the luminosity, contrast, shadow, reflection, and saturation were settled up at 80, 100, 0, 0, and 100%, respectively, using window 7 familial edition; see Fig. 1 for the flow chart.Figure 1

Schematic figure of the flowchart of imaging, labeling process and inclusion in the dataset.

Cannet A., Simon-Chane C., Akhoundi M., Histace A., Romain O., Souchaud M., Jacob P., Delaunay P., Sereno D., Bousses P., Grebaut P., Geiger A., de Beer C., Kaba D, & Sereno D. (2022). Wing Interferential Patterns (WIPs) and machine learning, a step toward automatized tsetse (Glossina spp.) identification. Scientific Reports, 12, 20086.

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Assessing Biofilm Formation Dynamics

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Liquid agar was prepared by autoclaving 1% tryptone (Teknova), 1% agar (Teknova) mixture. The liquid agar was cooled to 60˚C and Congo Red (EMD; final concentration 40 μg/mL) and Coomassie Blue (EMD; final concentration: 20 μg/mL) were added. TLCA stock solution, dissolved in MeOH, was vortexed for 2–3 min until clear. TLCA was added to liquid agar, different amounts were added to reach the final concentrations of 100 μM, 250 μM, and 1 mM TLCA. 60 mL of liquid agar mixture was poured into square plates (LDP, 10 cm x 10 cm x 1.5 cm) and left to solidify for ~18–24 h. Precultures of P. aeruginosa PA14 were grown in LB for 12–16 h at 37˚C while shaking at 250 rpm. Subcultures were prepared as 1:100 dilutions of precultures into fresh LB media and shaking for 2.5 h at 37˚C, at which point all subcultures had reached mid-exponential phase (optical density of ~0.4–0.6 at 500 nm). Morphology plates were dried for 20–30 min and 10 μL spots of subculture were spotted onto a morphology plate, with not more than four colonies per plate. Colony biofilms were grown at 25˚C and high humidity (+90%) for up to 5 d. Images were taken every 24 h with a Keyence VHX-1000 microscope.

Condren A.R., Kahl L.J., Boelter G., Kritikos G., Banzhaf M., Dietrich L.E, & Sanchez L.M. (2020). Biofilm inhibitor taurolithocholic acid alters colony morphology, specialized metabolism, and virulence of Pseudomonas aeruginosa. ACS infectious diseases, 6(4), 603-612.

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Evaluating Skin Thickness and Epidermal Morphology

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Skin samples were isolated at week six and fixed with 10% formalin and embedded in paraffin. Sections of paraffin embedded samples were stained with hematoxylin and eosin (H&E). Skin samples were observed and measured with a digital VHX-1000 microscope (Keyence, Osaka, Japan). Skin thickness was evaluated in H&E-stained sections as the distance from the bottom of the stratum corneum to the deepest portion of the reticular dermis where the adipose tissue or the muscle was reached. The epidermal thickness in H&E-stained sections was also evaluated by the distance between the top of the basement membrane and the bottom of the stratum corneum. Three randomly selected fields from each section were measured.

Shibata S., Kuwahara A., Sakaki-Yumoto M., Kawaguchi M., Ishii T, & Honma Y. (2022). Effects of the dipeptide L-glutamic acid-L-tryptophan on dermatitis in mice and human keratinocytes. Heliyon, 8(1), e08729.

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