Aunps

Manufactured by BBI Solutions

Sourced in United Kingdom

AuNPs are gold nanoparticles, spherical in shape, with a size range of 5-100 nanometers. They exhibit unique optical, electrical, and catalytic properties due to their nanoscale dimensions.

Automatically generated - may contain errors

Lab products found in correlation

9 protocols using aunps

AuNPs-based Thrombin Aptamer Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

AuNPs (40 nm in diameter) were obtained from BBI solutions (Cardiff, UK). Thrombin binding aptamer (Th-apt, 5′-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′) was purchased from Eurofins Genomics (Tokyo, Japan). 22 2-Melcaptethanol (2-ME) and methyl orange were obtained from Wako (Osaka, Japan). 3-Aminopropyltriethoxysilane (APTES) and bovine serum albumin (BSA) were obtained from Tokyo Chemical Industry (Tokyo, Japan) and Bio RAD (Hercules, CA, USA), respectively. Slide glass and cover glass were purchased from Matsunami Glass Industry (Osaka, Japan).

Yano Y., Nisougi M., Yano-Ozawa Y., Ohguni T., Ogawa A., Maeda M., Asahi T, & Zako T. (2019). Detection of Gold Nanoparticles Aggregation Using Light Scattering for Molecular Sensing. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 35(6).

+ Open protocol

+ Expand

Synthesis and Characterization of AuNP Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neutravidin was purchased from Thermo-Scientific. Peptide 1 with the sequence CALNN and peptide 2 with the sequence CALNN(PEG) 4 SNKTRIDEANQRATKLLGSGDDGDDGDD were purchased from GL Shanghai, China. AuNPs of size 40nm, 60nm and 80nm were purchased from BBI solutions. Hydrogen peroxide 30%wt was purchased from VWR chemicals, Poland. Gold (III) chloride trihydrate (99.9%), sodium citrate tribasic dehydrate (99%), ascorbic acid (99%), silver nitrate (99%), cetyl trimethylammonium bromide (96%) (CTAB), chitosan (low molecular weight), acetic acid (99.7%), 3-Mercaptopropionic acid (99%) (MPA), hydroxylamine hydrochloride (99%), sodium borohydride (98%), sodium chloride (99%) and glycerol (99%) were purchased from Sigma Aldrich. Hydrochloric acid (37%) was purchased from Merck Millipore, Singapore. The synthesis of the polypeptide JR2EC with the sequence (NAADLEKAIEALEKHLEAKGPCDAAQLEKQLEQAFEA FERAG) has been described elsewhere. 28 MMP7 human recombinant, E. Coli was purchased from Merck Chemicals.

Chen P., Liu X., Goyal G., Tran N.T., Shing Ho J.C., Wang Y., Aili D, & Liedberg B. (2018). Nanoplasmonic Sensing from the Human Vision Perspective. Analytical chemistry, 90(7).

+ Open protocol

+ Expand

Thermally Controlled Plasmonic Nanostructure Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 3 mm–by–3 mm Au film (70 nm) on Si substrate was immersed in 20 mM OS-1 (Fig. 1A) or OS-2 (Fig. 4A)/chloroform solution overnight, followed by rinsing with pure chloroform (99%; Yonghua Chemical Co. Ltd.) and dried quickly. Au NPs (5 μl; 80 nm; BBI Solutions) were drop-casted on the film for 20 min and blow-dried using nitrogen. For solvent treatment, pure chloroform, MeOH (99.8%; Hushi Co. Ltd.) and dichloromethane (99.9%; Aladdin) mixed at the ratio of 1:8 and 1:4, and pure MeOH were used. For temperature-induced disassembly/assembly, the sample was immersed into hot chloroform (60°C) for 30 s and room temperature chloroform for 3 hours, respectively. All the samples were vacuum-dried to remove any residue solvent before taking DF scattering spectra.

Zhang C., Li D., Zhang G., Wang X., Mao L., Gan Q., Ding T, & Xu H. (2022). Switching plasmonic nanogaps between classical and quantum regimes with supramolecular interactions. Science Advances, 8(5), eabj9752.

+ Open protocol

+ Expand

Fluorescent Probes Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

AuNPs (5 nm in water, EM.GC5) were purchased from BBI Solutions (Newport, Wales, UK). 3-(3,4- Dihydroxyphenyl) propionic acid (DPA), bPEI (25 kDa), and RITC were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein-conjugated HP (H7482) was purchased from Invitrogen (Carlsbad, CA, USA).

Yi S.W., Park J.S., Kim H.J., Lee J.S., Woo D.G, & Park K.H. (2019). Multiply clustered gold-based nanoparticles complexed with exogenous pDNA achieve prolonged gene expression in stem cells. Theranostics, 9(17), 5009-5019.

+ Open protocol

+ Expand

Colorimetric and Fluorescent AuNP Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

AuNPs (20 and 40 nm) were obtained from BBI Solutions (Cardiff, UK). All oligonucleotides (Probe-A, Probe-B, ssDNA-15, ssDNA-20, agDNA-15, agDNA-20, RNA-15, NA, NB, and MB) shown in Figure S1 were purchased from FASMAC (Kanagawa, Japan). T7 RNA polymerase was purchased from Takara Bio Inc. (Shiga, Japan). 96-7 DNA polymerase and RNase inhibitor were purchased from Nippon Gene (Tokyo, Japan). Deoxynucleotide solution mix (dNTPs) and ribonucleotide solution mix (rNTPs) were purchased from New England Biolabs Inc. (Ipswich, ME, USA). Human α-thrombin was purchased from Funakoshi (Tokyo, Japan). Transferrin, human immunoglobulin G (IgG), human serum albumin (HSA), and bovine serum albumin (BSA) were purchased from Merck (Munich, Germany). NAP-5 columns were provided with the oligonucleotides. Absorption spectra and fluorescence intensities were measured using microplate reader (TECAN, Zürich, Switzerland). All photographs were taken using a digital camera (SONY, Tokyo, Japan).

Muto Y., Hirao G, & Zako T. (2021). Transcription-Based Amplified Colorimetric Thrombin Sensor Using Non-Crosslinking Aggregation of DNA-Modified Gold Nanoparticles. Sensors (Basel, Switzerland), 21(13), 4318.

+ Open protocol

+ Expand

AuNPs-based IgA Immunoassay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents used were of guaranteed grade and used without further purification. All inorganic salts, 1-Ethyl-3-(3dimethylaminopropyl) carbodiimide ·HCl (EDC), N-hydroxysuccinimide (NHS), polyethylene glycol (Mw is 20,000), trehalose dihydrate, and pH 7.5 D-PBS (-) were purchased from Fujifilm Wako Pure Chemicals (Osaka, Japan). AuNPs with diameters of 60 nm were purchased from BBI Solutions (Cardiff, UK). Anti-IgA (α), Human, Goat-Poly, A80-102A, and Purified Human IgA, P80-102 were purchased from Bethyl Laboratory (Montgomery, AL, USA). Bovine serum albumin (BSA) was purchased from Jackson Immunoresearch (West Grove, PA, USA). All water used in this study was Milli-Q water (18.3 MΩ cm).

Osaki S., Saito M., Nagai H, & Tamiya E. (2024). Surface Modification of Screen-Printed Carbon Electrode through Oxygen Plasma to Enhance Biosensor Sensitivity. Biosensors, 14(4), 165.

+ Open protocol

+ Expand

Colloidal Au Nanoparticles Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Colloidal suspension of 100 nm diameter AuNPs was purchased from BBI solutions (UK).
Poly(methyl methacrylate) (PMMA) with a nominal molecular weight of 100 kDa, used for creating the replica template from the Si master, was purchased from Polysciences, Inc.
(Warrington, PA, USA). Potassium hexacyanoferrate (II) trihydrate and potassium hexacyanoferrate (III) used for electro-/photoelectro-chemistry and biphenyl-4-thiol used for single particle SERS were all purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Lee J.B., Walker H., Li Y., Nam T.W., Rakovich A., Sapienza R., Jung Y.S., Nam Y.S., Maier S.A, & Cortés E. (2020). Template Dissolution Interfacial Patterning of Single Colloids for Nanoelectrochemistry and Nanosensing. ACS nano, 14(12).

+ Open protocol

+ Expand

Thiol-modified Oligonucleotide Immobilization on Gold

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thiol-modified custom-designed oligonucleotides (sequences are presented in Table 1), dithiothreitol (DTT), 6-mercapto hexanol (MHOH), and NAP-10 columns were obtained from Sigma-Aldrich (Woodlands, TX, USA). AuNPs(50 nm diameter) were purchased from BBI Solutions (Cardiff, UK). Human serum samples were purchased from Fitzgerald Industries International Inc. (North Acton, MA, USA). SpotReady-16 gold spotted glass microarray chips (spot size 1 mm diameter, SPR-1000-016) were obtained from GWC Technologies (Madison, WI, USA). Gold disc infused quartz crystals (gold diameter 0.2 inch) used for the QCM were purchased from International Crystal Manufacturing Co, Inc.(Oklahoma City, OK, USA). All other chemicals were of high-purity analytical grade. To prepare all solutions, ultrapure distilled water (DNAse and RNAse free) purchased from Life Technologies (Carlsbad, CA, USA) was used.
The hybridization sequences for probe, target, and control are highlighted. Base pair mismatches of the control with respect to the target are highlighted in red. The addition of Poly-T₅₀ segments to the target and control sequences provides stability and flexibility to the strands.^{29 (link)}

Premaratne G., Al Mubarak Z.H., Senavirathna L., Liu L, & Krishnan S. (2017). Measuring Ultra-low Levels of Nucleotide Biomarkers Using Quartz Crystal Microbalance and SPR Microarray Imaging Methods: A Comparative Analysis. Sensors and actuators. B, Chemical, 253, 368-375.

+ Open protocol

+ Expand

Synthesis and Characterization of Halogenated Nucleobases

Check if the same lab product or an alternative is used in the 5 most similar protocols

Guanine
(Gua), 8-bromoGuanine (BrGua), silver
nitrate (AgNO₃), and trisodium citrate were purchased from
Sigma-Aldrich (Germany). 8-Bromoadenine (BrAde) was purchased from
Carbosynth (U.K.). Sodium hydroxide (NaOH) was purchased from Merck.
AuNPs (diameter 40 nm) were purchased from BBI solutions. Si-wafers
were purchased from CrysTec GmbH (100 mm, p-Typ). All chemicals were
used as received without further purification. Milli-Q grade water
was used for synthesis and experimental work.

Dutta A., Schürmann R., Kogikoski S J.r., Mueller N.S., Reich S, & Bald I. (2021). Kinetics and Mechanism of Plasmon-Driven Dehalogenation Reaction of Brominated Purine Nucleobases on Ag and Au. ACS Catalysis, 11(13), 8370-8381.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!