Epc 7 patch clamp amplifier

Manufactured by HEKA Elektronik

Sourced in Germany

The EPC 7 is a patch-clamp amplifier designed for electrophysiological measurements. It provides the necessary electrical interface between the biological preparation and the data acquisition system.

Automatically generated - may contain errors

Lab products found in correlation

Two stage vertical puller, by HEKA Elektronik (2 mentions) Prism 5, by GraphPad (2 mentions) Labview 2015, by National Instruments (1 mentions) Axioscope, by Zeiss (1 mentions) Pulse pulsefit software, by HEKA Elektronik (1 mentions) Pclamp6, by Molecular Devices (1 mentions)

5 protocols using epc 7 patch clamp amplifier

Whole-cell patch-clamp of glycine and GABA currents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glycine-activated currents (I_Gly) and
GABA-activated currents (I_GABA) in isolated
neurons were induced by a step application
of an agonist for 600–1000 ms with 30–40 s intervals
through a glass capillary, 0.1 mm in diameter, which could be rapidly
displaced laterally. Transmembrane currents were recorded using a
conventional patch-clamp technique in the whole-cell configuration.
Patch-clamp electrodes had a tip resistance of ∼2 MΩ.
The solution in the recording pipet contained the following (in mM):
40 CsF, 100 CsCl, 0.5 CaCl₂, 5 EGTA, 3 MgCl₂, 4 NaATP, 5 HEPES, pH 7.3. The composition of the extracellular
solution was as follows (in mM): 140 NaCl, 3 KCl, 3 CaCl₂, 3 MgCl₂, 10 d-glucose, 10 HEPES hemisodium,
pH 7.4. The speed of perfusion was 0.6 mL/min. Recording of the currents
was performed using an EPC7 patch-clamp amplifier (HEKA Elektronik,
Germany). The holding potential was maintained at −70 mV. Transmembrane
currents were filtered at 3 kHz, stored, and analyzed with IBM-PC
computer, using homemade software.

Solntseva E.I., Bukanova J.V., Kondratenko R, & Kudova E. (2023). Corticosteroids as Selective and Effective Modulators of Glycine Receptors. ACS Chemical Neuroscience, 14(17), 3132-3142.

+ Open protocol

+ Expand

Patch-Clamping Vacuolar Ion Transport

Check if the same lab product or an alternative is used in the 5 most similar protocols

Patch-clamp experiments on GFP-positive vacuoles were performed in the whole-vacuole configuration at RT, as described elsewhere [84] , using an EPC-7 patch clamp amplifier (HEKA Elektronik, Lambrecht, Germany). Patch pipettes were pulled from thinwalled borosilicate glass (Clark Electrochemical Instruments, Pangbourne, Reading, UK), with a tip resistance of 3 -3.5 MOhm.
The pipette solution contained (in mM): 100 KCl, 3 MgCl 2 , 20 MES, adjusted to pH 5.5 (with KOH) and 480 mOsm (with D-sorbitol). Bath solutions contained (in mM): 100 K-x, 2 EGTA, 3 MgSO 4 , 10 HEPES, adjusted to pH 7.2 (with KOH) and 480 mOsm (with D-sorbitol), where x was either gluconate, chloride or nitrate. High-resistance membrane seals were generally formed in VR solution. After the establishment of the whole-vacuole configuration, the bath solution was exchanged, and membrane currents were allowed to stabilize for 10-15 min. Bath solutions were exchanged by means of a gravity-driven perfusion system coupled to a peristaltic pump.
Membrane currents were elicited by 1 s voltage steps ranging from +80 to -80 mV in 20 mV decrements, followed a 500 ms voltage step to +50 mV. The holding potential was set to 0 mV.
Positive currents correspond to anions flowing from the lumen of the vacuole to the cytoplasmic side or to cations moving in the opposite direction.

Böhm J., Messerer M., Müller H.M., Scholz-Starke J., Gradogna A., Scherzer S., Maierhofer T., Bazihizina N., Zhang H., Stigloher C., Ache P., Al-Rasheid K.A.S., Mayer K.F.X., Shabala S., Carpaneto A., Haberer G., Zhu J.K, & Hedrich R. (2018). Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa. Current biology : CB, 28(19).

+ Open protocol

+ Expand

Patch-Clamp Measurement of Islet Cell Membrane Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols

The plasma membrane potential of a single cell within a microgripper-held islet or pseudoislet was measured using the patch-clamp technique. Pipettes were pulled from borosilicate glass (2 mm o.d., 1.4 mm i.d., Hilgenberg, Germany) by a two-stage vertical puller (HEKA-Electronics, Lambrecht, Germany) and had resistances between 3 and 6 MΩ when filled with solution. The measurements were performed using an EPC 7 patch-clamp amplifier (HEKA-Electronics), Labview 2015 software, and a PCI 7833 data acquisition board (National Instruments Germany, München, Germany). The data were stored on a hard disk and analyzed offline using GraphPad Prism5 software (GraphPad, LaJolla, CA, USA). A slow bath perfusion system was used, and all experiments were performed at room temperature (20–22 °C). For the measurements of the membrane potential in the perforated patch mode [15 (link),16 (link)], the pipette solution contained (mM): 10 KCl; 10 NaCl; 70 K₂SO₄; 7 MgCl₂; and 5 HEPES, with a pH of 7.15, plus 125 µg/mL of the pore-forming agent, nystatine (2.5% DMSO final concentration). The extracellular bath solution contained (mM): 140 NaCl; 5.6 KCl; 1.2 MgCl₂; 2.6 CaCl₂; 10 HEPES; and 1 glucose, with a pH of 7.4.

Früh E., Bütefisch S., Gursky B., Brüning D., Leester-Schädel M., Dietzel A, & Rustenbeck I. (2022). An Immersible Microgripper for Pancreatic Islet and Organoid Research. Bioengineering, 9(2), 67.

+ Open protocol

+ Expand

Amperometric Measurement of Dopamine Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols

To monitor single-cell secretory activity, dopamine secretion from glomus cells in CB slices was measured by amperometry.^{23 (link)} Secretory events were recorded with a 10 μm carbon fiber electrode polarized to +750 mV (to favor dopamine oxidation) using an external voltameter connected to the EPC-7 amplifier. Amperometric currents were recorded with an EPC-7 patch-clamp amplifier (HEKA Electronics, Lambrecht/Pfaltz). The signal was filtered at 100 Hz and digitized at 250 Hz before storage on computer. Data acquisition and analysis were carried out with an ITC-16 interface (Instrutech Corporation) and PULSE/PULSEFIT software (HEKA Electronics). For the experiments, a slice was transferred to the recording chamber of an upright microscope (Axioscope, Zeiss) and continuously perfused with extracellular solution (see the “Recording Solutions” section). The secretion rate (given in fC/min or pC/min) was calculated as the amount of charge transferred to the recording electrode during a given period of time. Experiments were performed at ∼35°C

Colinas O., Mombaerts P., López-Barneo J, & Ortega-Sáenz P. (2024). Carotid Body Function in Tyrosine Hydroxylase Conditional Olfr78 Knockout Mice. Function, 5(3), zqae010.

+ Open protocol

+ Expand

Patch-Clamp Profiling of Islet Cell Membrane Potentials

Check if the same lab product or an alternative is used in the 5 most similar protocols

The plasma membrane potential of 1-day- or 2-day-cultured single islet cells was measured using the patch clamp technique in the perforated patch configuration. Pipettes were pulled from borosilicate glass (2 mm o.d., 1.4 mm i.d., Hilgenberg, Germany) by a two-stage vertical puller (HEKA-Electronics, Lambrecht, Germany) and had resistances between 3 and 6 MΩ when filled with solution. The measurements were performed using an EPC 7 patch clamp amplifier (HEKA-Electronics) in the current clamp mode and pClamp 6.03 software (Axon Instruments, Foster City, CA, USA). The data were stored on a hard disk and analysed offline using GraphPad Prism5 software (GraphPad, LaJolla, CA, USA). A slow bath perfusion system was used, and all experiments were performed at room temperature (20–22 °C). The pipette solution contained the following (mM): 10 KCl, 10 NaCl, 70 K₂SO₄, 7 MgCl₂, and 5 HEPES, pH 7.15, plus 125 µg/mL of the pore-forming agent nystatine (2.5% DMSO final concentration). The extracellular solution contained (mM) 140 NaCl, 5.6 KCl, 1.2 MgCl₂, 2.6 CaCl₂, 10 HEPES, and 1 glucose, pH 7.4.

Müller M., Walkling J., Seemann N, & Rustenbeck I. (2023). The Dynamics of Calcium Signaling in Beta Cells—A Discussion on the Comparison of Experimental and Modelling Data. International Journal of Molecular Sciences, 24(4), 3206.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!