Histone h3 k27m

Manufactured by Merck Group

Histone H3-K27M is a type of histone protein that contains a lysine-to-methionine substitution at the 27th amino acid position. It is commonly used in research applications as a molecular marker or target for studying epigenetic regulation and chromatin structure.

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Lab products found in correlation

Idh1 r132h dia h09, by Dianova (1 mentions) Ki 67 30 9, by Roche (1 mentions)

Close spelling variants of this product

Histone H3 Histones

3 protocols using histone h3 k27m

Immunohistochemical Analysis of Glioma Samples

Cited 2 times

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IHC was performed on selected sections, as described [12 (link), 15 (link)]. The following primary antibodies were used: histone H3-K27M (Millipore/Sigma, Burlington, MA), IDH1-R132H (DIA-H09, Dianova, Hamburg, Germany), p53 (DO-7), vimentin (V9), Ki-67 (30-9) (Roche/Ventana Medical Systems Inc., Tucson, AZ), Olig-2 (387M-15), GFAP (EP672Y) (Ventana/ Cell Marque, Rocklin, CA).

Georgescu M.M., Islam M.Z., Li Y., Traylor J, & Nanda A. (2021). Novel targetable FGFR2 and FGFR3 alterations in glioblastoma associate with aggressive phenotype and distinct gene expression programs. Acta Neuropathologica Communications, 9, 69.

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Immunohistochemical Analysis of Tumor Specimens

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Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections at the CUMC Department of Pathology and Cell Biology Laboratory. Histological analysis of tumor specimens was done with Hematoxylin & Eosin (H&E) staining. Immunoperoxidase staining was done on paraffin-embedded sections using antibodies to Ki67, IDH1-R132H, ATRX, p53 (all from Ventana, Tucson, AZ), and histone H3 K27M (Millipore Sigma, Billerica, MA). A neuropathologist (GZ) evaluated the histology. Pathology reports were reviewed for additional histological analyses performed during routine clinical pathological evaluation at the time of initial diagnosis.

D’Amico R.S., Zanazzi G., Wu P., Canoll P, & Bruce J.N. (2018). Pineal region glioblastomas display features of diffuse midline and non-midline gliomas.. Journal of neuro-oncology, 140(1), 63-73.

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Immunohistochemical Analysis of Glioma Markers

Cited 2 times

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IHC was performed on selected sections, as described [20 (link), 22 (link)]. Primary antibodies were: histone H3-K27M (Millipore/Sigma, Burlington, MA), IDH1-R132H (DIA-H09, Dianova, Hamburg, Germany), p53 (DO-7), vimentin (V9), Ki-67 (30–9) (Roche/Ventana, Tucson, AZ), Olig-2 (387 M-15), GFAP (EP672Y) (Ventana/Cell Marque, Rocklin, CA).

Georgescu M.M., Islam M.Z., Li Y., Circu M.L., Traylor J., Notarianni C.M., Kline C.N, & Burns D.K. (2020). Global activation of oncogenic pathways underlies therapy resistance in diffuse midline glioma. Acta Neuropathologica Communications, 8, 111.

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