The number of Purkinje cells was counted, and the length of the PCL was measured in every lobule of the cerebellum by using the BZ-II Analyzer software (Keyence). Data were expressed as the mean cell number per millimeter (length of PCL) ± SEM in every lobule. The number of Iba1+ cells was counted, and the areas of the ML and the PCL (designated as ML/PCL) or the granule cell layer (GCL) and the white matter (WM; designated as GCL/WM) were measured in every lobule of the cerebellum by using the BZ-II Analyzer software (Keyence). Data were expressed as the mean cell number per square millimeter (area of ML/PCL or GCL/WM) ± SEM.
Bz 2 analyzer software
Manufactured by Keyence
Sourced in Japan, Germany
The BZ-II Analyzer software is a tool designed for the analysis of data collected from Keyence's BZ-II series of digital microscopes. The software provides functionalities for displaying, processing, and analyzing the images and measurements obtained from the microscopes. It offers basic image manipulation and measurement features to support users in their microscopic analysis tasks.
Automatically generated - may contain errors
Lab products found in correlation
Bz 9000, by Keyence (18 mentions)
Bz 9000 microscope, by Keyence (13 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (7 mentions)
Biorevo bz 9000 microscope, by Keyence (6 mentions)
Bz 9000 fluorescence microscope, by Keyence (5 mentions)
Paraformaldehyde (pfa), by Fujifilm (5 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (5 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Biorevo bz 9000, by Keyence (3 mentions)
Bz 9000 fluorescent microscope, by Keyence (3 mentions)
Bz x710, by Keyence (3 mentions)
Hoechst 33342, by Thermo Fisher Scientific (3 mentions)
Triton x 100, by Merck Group (3 mentions)
Lsm 710 confocal microscope, by Zeiss (3 mentions)
Bz 9000 all in one fluorescence microscope, by Keyence (2 mentions)
Bisbenzimide h33342 fluorochrome trihydrochloride hoechst, by Nacalai Tesque (2 mentions)
Blocking one, by Nacalai Tesque (2 mentions)
Histofix solution, by Carl Roth (2 mentions)
Bz 9000e series microscope, by Keyence (2 mentions)
Bz x700, by Keyence (2 mentions)
96 protocols using bz 2 analyzer software
1
Purkinje Cell and Microglial Quantification
2
Immunofluorescence Analysis of DNA Damage
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At different time points after irradiation (1–24 h), cells were spun on cover slips, fixed with 3.7% PFA and permeabilized with 0.5% Triton followed by washing and blocking steps with PBS and 5% goat serum in PBS. Cells on cover slips were immunostained with primary antibodies anti-γH2AX (Ser139, clone JBW301, Millipore), anti-53BP1 rabbit NB100-304 (Novus Biologicals, Littleton, CO, USA) and with Alexa Fluo®555-conjugated secondary antibodies (Invitrogen). Nuclear counter staining was performed with DAPI and cover slips were mounted with VectaShield mounting media (Vector Labs, Burlingame, CA, USA). Immunofluorescence signals were visualized by an Olympus BX51 epifluorescence microscope equipped with an Olympus XC10 camera and acquired images automatically analyzed by CellF2.5_analysis software including the mFIP software (Olympus Soft Imaging System, Münster, Germany) or by Keyence BZ-II Analyzer software (Keyence, Neu-Isenburg, Germany).
3
DNA Damage Repair Analysis in Lymphocytes
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For in situ analysis of DSB repair by HR, we performed immunofluorescence microscopic analysis of the DNA damage marker γH2AX and the recombinase RAD51 in PBLs after exposure to 2 Gy of ionizing radiation (IR) as previously described (Gatz et al., 2016 ; Obermeier et al., 2016 ). Briefly, PBLs were harvested by cytospinning at the indicated time points postirradiation, fixed with 3.7% formaldehyde followed by permeabilization with 0.5% TritionX‐100. Primary antibodies were directed against γH2AX (Ser139, clone JBW301, Millipore, Billerica, MA, USA) and RAD51 (H‐92, Santa Cruz Biotechnology, Heidelberg, Germany), and the secondary antibodies were AlexaFluor488 and 555‐labeled (Invitrogen, Karlsruhe, Germany). Immunostained cells were mounted with VectaShield mounting media containing DAPI (Vector laboratories, Burlingame, CA, USA). Focal accumulations of 53BP1 and RAD51 in ≥97 DAPI‐stained nuclei from two independent slides were analyzed using a Keyence BZ‐9000 microscope equipped with Keyence BZ‐II Analyzer software (Keyence, Neu‐Isenburg, Germany).
4
3D LLC Cell Spheroid Imaging
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After spheroid formation (at 24 h after seeding), the agents under the study were added: D-g-PAAan, Dox and D-g-PAAan-Dox in a 7.4 μM Dox equivalent concentration. Then, 3D LLC cell spheroid were treated for 7 h and visualized with a fluorescence microscope Keyence BZ-9000 BIOREVO (Osaka, Japan) equipped with a red (λex = 480 nm, λem = 600 nm) filter and the respective acquisition software Keyence BZ-II Viewer (Osaka, Japan). The overlayed images were processed with the Keyence BZ-II Analyzer Software (Osaka, Japan).
5
Fluorescence Imaging of C60 in CCRF-CEM Cells
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Images of CCRF-CEM cells stained with DAPI, Dox, and FITC-labeled antibodies against C60, were viewed with a fluorescence microscope Keyence BZ-9000 BIOREVO (Osaka, Japan). The microscope was equipped with blue (λex = 377 nm, λem = 447 nm), green (λex = 472 nm, λem = 520 nm), and red (λex = 543 nm, λem = 593 nm) filters. The acquisition Keyence BZ-II Viewer Software (Osaka, Japan) was used. The merged images were processed with the Keyence BZ-II Analyzer Software (Osaka, Japan).
6
Myelinated Axon Analysis of Sciatic Nerve
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Twelve weeks postoperatively, the rats were given a lethal dose of isoflurane, perfused transcardially with 0.9% saline to flush the blood followed by 4% PFA in 0.1 M PBS, pH 7.4. The sciatic nerves were harvested and placed in 4% PFA for 24 hours and then parsed into three segments: a 3 mm-long segment taken 3 mm proximal to the SNG, a 3 mm-long segment taken in the center of the SNG, and a 3 mm-long segment taken 3 mm distal to the SNG. To view myelinated axons, the 3 mm-long segments were rinsed twice in 1× PBS, then placed in 2% osmium tetroxide in 1× PBS for 2 hours, before being dehydrated in ethanol and paraffin embedded (Di Scipio et al., 2008). The paraffin embedded segments were then sectioned transversally 5 µm thick, placed on slides and cover slipped with Permount. All sections were imaged under the same parameters at 20× on a Keyence BZ-9000 (Keyence Corporation, Itasca, IL, USA). An assessment of the myelinated axons was conducted using the Keyence BZ-II Analyzer software (Keyence Corporation) using the same threshold for all images. Only axons larger than 1 µm in diameter were analyzed.
7
Epididymal Adipocyte Characterization
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Epididymal fat was fixed in 10% neutral formalin, processed in paraffin blocks, sectioned to a thickness of 4 μm, and stained with haematoxylin and eosin. Slides were scanned using a BIOREVO BZ-9000-Generation-II microscope (KEYENCE Co.), and the sizes of 700–1500 adipocytes were measured using BZ-II-Analyzer software (version 2.2, KEYENCE Co.). The number of adipocytes per total area of counted adipocytes was determined as the cell density.
8
Alkaloid Effects on Tubulin-GFP in U2OS Cells
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α-Tubulin-GFP U2OS cells (1 × 105) were seeded in 24-well plates and grown for 24 h. Then, 200 μL different concentrations of alkaloids (IC80, IC50) were added into each well, and the cells were imaged under a Keyence BZ-9000 microscope (Keyence, Neu-Isenburg, Germany) after incubation for 2 h, 4 h, 24 h and 48 h. The images were analyzed using BZ-II Analyzer software (version 2.1, Keyence, Neu-Isenburg, Germany).
9
Placental Histology Analysis Protocol
10
Visualization of Cytoskeleton Organization
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Cells were seeded on cover slides and incubated over 24 h (37 °C, 5% CO2, humidified atmosphere). The culture medium was changed, and cells were treated for 5 s (A673, RD-ES) or 10 s (MNNG/HOS, U-2 OS) with CAP or carrier gas argon. After an incubation period of 4 h, the cells were washed with PBS and fixed with 1% paraformaldehyde (Carl Roth, Karlsruhe, Germany). Cells were permeabilized with Triton-X100 (0.3%; Carl Roth, Karlsruhe, Germany). Cells were stained for 20 min with Rhodamine conjugated Phalloidin (0.022 µM) (Thermo Fisher Scientific, Waltham, MA, USA) and Alexa Fluor 488 conjugated Deoxyribonuclease I (0.3 µM) (Thermo Fisher Scientific, Waltham, MA, USA) in the dark. DAPI (1.43 µM) (Thermo Fisher Scientific, Waltham, MA, USA) was added for the last 3 min of incubation. The fluorescence was recorded with BZ-9000 microscope and analyzed with BZ-II Analyzer software (KEYENCE, Neu-Isenburg, Germany). The ratio of the red to the green signal of each cell was calculated.
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