Hcx plan apo 63x 1.4 na

Cells grown during 5–7 days in shaken liquid BG11₀ NH₄⁺ medium or heterocyst preparations were observed and photographed with a Zeiss (Oberkochen, Germany) Axioscop microscope equipped with a Zeiss ICc1 digital camera. GFP fluorescence was analyzed by confocal microscopy. Samples from cultures of Anabaena sp. strain CSMI21 or the wild-type PCC 7120 as a control grown in bubbled cultures with BG11 or BG11₀ medium were visualized using a Leica HCX PLAN-APO 63X 1.4 NA oil immersion objective attached to a Leica TCS SP2 confocal laser-scanning microscope. GFP was excited using 488-nm irradiation from an argon ion laser. Fluorescence emission was monitored by collection across windows of 500–520 nm (GFP imaging) and 630–700 nm (cyanobacterial autofluorescence). Under the conditions used, optical section thickness was about 0.4 μm. GFP fluorescence intensity was analyzed using ImageJ 1.43 m software (http://imagej.nih.gov/ij/).

Anabaena filaments on solid medium were analyzed by confocal microscopy using a Leica HCX PLAN-APO 63X 1.4 NA oil immersion objective attached to a Leica TCS SP2 confocal laser-scanning microscope. GFP was excited at 488 nm using an argon ion laser. Fluorescent emission was monitored by collection across windows of 500–540 nm (GFP imaging) and 630–700 nm (cyanobacterial autofluorescence).