Goat anti chicken igy

Manufactured by Abcam

Goat anti-chicken IgY is a secondary antibody that specifically binds to chicken IgY immunoglobulins. It is commonly used in immunoassays and other applications that require the detection or isolation of chicken antibodies.

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HRP-conjugated goat anti-chicken IgY (6 citations) Goat anti-chicken IgY Alexa Fluor 488 (4 citations) Goat anti-chicken IgY- DyLight 488 (3 citations) Alexa Fluor 647 goat anti-chicken IgY H&L (2 citations) Alexa 405 goat anti-chicken immunoglobulin Y (IgY H&L) (2 citations) Horseradish peroxidase-conjugated goat-anti-chicken IgY antibody (2 citations) Goat anti-chicken IgY-horseradish peroxidase (HRP) antibodies (2 citations) Goat Anti- Chicken IgY H&L-Alexa Fluor® 488 (2 citations) Alexa Fluor 647-conjugated goat anti-chicken IgY (2 citations) Goat anti-chicken IgY AlexaFluor 647 (2 citations)

5 protocols using goat anti chicken igy

Immunofluorescent Labeling of Brain Slices

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Brain slices were blocked in 4% (v/v) normal goat serum (Abcam Inc., Cambridge, UK) with 0.3% (v/v) Triton X-100 (Sigma-Aldrich, Saint Louis, MO, USA) in 1× PBS with 0.1% sodium azide (Alfa Aesar) for one hour. Slices were then incubated overnight with primary antibodies targeting neuronal nuclei (NeuN), astrocytes (GFAP), and activated microglia/macrophages (CD68) (Table 1) at 4 °C in a buffer solution containing only 0.1% (v/v) Triton X-100.
The following day, slices were washed and incubated for one hour in blocking solution with secondary antibodies, goat anti-rabbit IgG (TRITC), goat anti-mouse IgG (Alexa Fluor 488), goat anti-chicken IgY (Alexa Fluor 647), at 1:1000 dilution, and DAPI (0.6 μM) (Abcam Inc.). Slices were subsequently washed and mounted on glass slides with Fluoromount aqueous mounting medium (Sigma-Aldrich).

Stiller A.M., Usoro J., Frewin C.L., Danda V.R., Ecker M., Joshi-Imre A., Musselman K.C., Voit W., Modi R., Pancrazio J.J, & Black B.J. (2018). Chronic Intracortical Recording and Electrochemical Stability of Thiol-ene/Acrylate Shape Memory Polymer Electrode Arrays. Micromachines, 9(10), 500.

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Immunoblotting for Malaria Parasite Proteins

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Protein samples were electrophoresed on a NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred onto a nitrocellulose membrane using iBlot system (Invitrogen). The membrane was subsequently blocked using 1% casein in 1x PBS and incubated with primary antibody solutions with following dilutions: mouse anti-HA (Sigma, 1:500), rabbit anti-HSP101 (r950, 1:500), rabbit anti-PTEX150 (r741, 1:500), mouse anti-EXP2 (5–10 μg/mL), rabbit anti-EXP2 (r1167, 1:1000), chicken anti-FLAG (Abcam,1:2000), mouse anti-FLAG (10 μg/mL), rabbit anti-Nluc (12.5 μg/mL), rabbit anti-plasmepsin V (1:2000), rabbit anti-GAPDH (1:2000), rabbit anti-SERA5 (1:1000) for 1 hour at RT or overnight at 4°C. Secondary antibodies were incubated for 1–2 hours in following dilutions: Goat anti-rabbit Alexa Fluor Plus 700 and 800, Goat anti-mouse Alexa Fluor Plus 700 and 800 (Invitrogen 1:10,000), and horseradish peroxidase-conjugated Goat anti-chicken IgY (Abcam, 1:10,000). For enhanced chemiluminescence detection, membranes were probed with SuperSignal West Pico PLUS substrate (Pierce). All imaging and densitometry analysis were performed using Odyssey Fc system (LI-COR).

Gabriela M., Matthews K.M., Boshoven C., Kouskousis B., Jonsdottir T.K., Bullen H.E., Modak J., Steer D.L., Sleebs B.E., Crabb B.S., de Koning-Ward T.F, & Gilson P.R. (2022). A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell. PLoS Pathogens, 18(2), e1009977.

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Immunohistochemical Characterization of Neuronal Cells

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Immunohistochemistry was performed on paraformaldehyde (4%) fixed cell cultures, paraffin sections (5 µm) and cryosections (10 µm) as described elsewhere [33] (link). The following primary antibodies were used: rabbit anti-Neural Class (III) beta-Tubulin (1∶2,000; TUJ1, Covance, Münster, Germany), rabbit anti-Nitric Oxide Synthase (1∶5,000; R&D, Las Vegas, USA), rabbit anti-protein gene product 9.5 (1∶800; AbD Serotec, Oxford, United Kingdom), rabbit anti-glial fibrillary acidic protein (1∶500; Dako, Glostrup, Danmark), rabbit anti-S100β (1∶200; abcam, Cambridge, Great Britain), mouse-anti human neuronal protein Hu c/d (1∶50; Invitrogen), chicken anti-green fluorescent protein (1∶500; abcam) and rat-anti BrdU (1∶50; AbD Serotec). Detection of the primary antibodies was performed with the following fluorochrome-linked secondary antibodies: goat anti-rabbit IgG Cy3 (1∶400; Invitrogen), goat anti-rat Cy3 (1∶400; Invitrogen), goat anti-rabbit IgG Cy5 (1∶500; Invitrogen) and goat anti-chicken IgY, Dylight 488 (1∶500; abcam). For identifying biocytin filled cells Cy³-conjugated streptavidin antibody (1∶500, Jackson ImmunoResearch, United Kingdom) was used.
Cell nuclei were stained with 4′-6-diamidino-2-phenylinodole (DAPI) solution (200 ng/mL; Invitrogen).

Dettmann H.M., Zhang Y., Wronna N., Kraushaar U., Guenther E., Mohr R., Neckel P.H., Mack A., Fuchs J., Just L, & Obermayr F. (2014). Isolation, Expansion and Transplantation of Postnatal Murine Progenitor Cells of the Enteric Nervous System. PLoS ONE, 9(5), e97792.

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Western Blot Protein Analysis Protocol

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Neuronal or HEK293T cultures were rinsed in PBS, scraped, and homogenized in ice-cold radioimmunoprecipitation lysis buffer (150 mM NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, and 50 mM Tris-HCl, pH 8.0) with protease inhibitor cocktail (cOmplete; Roche) and phosphatase-inhibitor cocktail (PhosSTOP; Roche). Protein concentrations were determined using DC Protein Assay kit (Bio-Rad Laboratories, Inc.). Samples were loaded in Laemmli sample buffer (63 mM Tris-HCl pH 7, 2% SDS, 20% glycerol, 5% β-mercaptoethanol, and bromophenol blue), separated using SDS-PAGE, and transferred to nitrocellulose membrane (Hybond ECL; GE Healthcare). Blots were probed with the appropriate primary antibodies (see Antibodies) and HRP-conjugated donkey anti–rabbit (GE Healthcare) or goat anti–chicken IgY (Abcam) antibodies and then developed with ECL Plus (GE Healthcare) and visualized with LAS-3000 (Fujifilm). Band densities were analyzed with AIDA imaging software (Raytest).

Llano O., Smirnov S., Soni S., Golubtsov A., Guillemin I., Hotulainen P., Medina I., Nothwang H.G., Rivera C, & Ludwig A. (2015). KCC2 regulates actin dynamics in dendritic spines via interaction with β-PIX. The Journal of Cell Biology, 209(5), 671-686.

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Immunohistochemical Analysis of Bone Marrow

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After fixation, samples were decalcified in 20% EDTA at 4 C for 1 week. Samples were embedded in OCT for frozen sectioning. 10mm sections were obtained. Sections were incubated with primary antibody at 4 C O/N. The following primary antibodies were used in this study: Anti-CD44, Anti-CD73, Anti-CD146, Anti-Periostin, Anti-Col I, Anti-Sp7, Anti-ß3-tublin, Anti-aSMA, Anti-LepR, Anti-ß-gal, Anti-SOST, Anti-Perilipin-1, Anti-CD31, Anti-CD34, and Anti-Nestin, Anti-NG2, Anti-PDGFRa, Anti-SHH. The secondary antibodies included goat anti-mouse (Invitrogen A11029, 1:100), anti-rat (Invitrogen A11006), anti-rabbit (Invitrogen A11034) IgG conjugated to Alexa 488, and goat anti-chicken IgY conjugated to AlexaFluo 488 (Abcam ab150169).
GS-IB4 (Isolectin GS-IB4 from Griffonia Simplicifolia) has been widely used for labeling endothelial cells in various organs including long bone marrow, lung, heart and retina (Epah et al., 2018; Hooper et al., 2009; Xu et al., 2018; Ohle et al., 2012; Arnold et al., 2012) . For periodontium vasculature staining, mouse samples were fixed with 4% PFA overnight and decalcified in 20% EDTA for one week. Samples were embedded in OCT and 20mm sections were obtained. Sections were incubated with GS-IB4 conjugated with Alexa-Fluo488 (Invitrogen) at 1:100 dilution at 4 CC O/N. Sections were then briefly washed with PBS and counterstained with DAPI for imaging.

Men Y., Wang Y., Yi Y., Jing D., Luo W., Shen B., Stenberg W., Chai Y., Ge W.P., Feng J.Q, & Zhao H. (2020). Gli1+ Periodontium Stem Cells Are Regulated by Osteocytes and Occlusal Force. Developmental cell, 54(5).

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